粉尘螨过敏原Derf21的生物信息学分析  

Bioinformatics Analysis of Dust Mite Allergen Der f21

作　　者：林建立[1] 巴金戈 刘志刚[1] 杨平常[1] 刘晓宇[1] LIN Jian-li;BA Jin-ge;LIU Zhi-gang;YANG Ping-chang;LIU Xiao-yu

机构地区：[1]深圳大学医学院过敏反应和免疫学研究所,广东深圳518060

出　　处：《南昌大学学报（医学版）》2016年第3期1-5,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(91442118;31400786);广东省科技计划社会发展项目(2013B03180002);广东省对外科技合作项目(2013B051000088);广东省公益研究与能力建设专项资金(2013B031800023);深圳市科技计划国际科技合作项目(GJHZ20130408174112021);深圳市科技计划基础研究项目(JCYJ20140828163633992)

摘　　要：目的运用生物信息学方法对粉尘螨过敏原Derf21特征进行分析。方法采用dUStalW2进行同源性分析、MEGA5工具包构建系统进化树、ProtParamTools预测其理化性质、KinasePhos软件预测磷酸化位点。采用PSIPRED预测其二级结构、SWISS-MODEL预测其三级结构。用DNAStar对Derf21的B细胞抗原表位进行预测。利用网络服务器IEDB内相关软件、Preprod、SYFPEITHI、NetMHCII2.2Server和NetMHCIIpan3.0Sever对Derf21蛋白T细胞抗原表位进行预测。结果粉尘螨基因组测序发现本实验室克隆的Derf21基因与Gen-Rank上公布的屋尘螨DerP21(GenBank:ABC73706.1)同源性为71%。系统进化树结果显示：粉尘螨与屋尘螨、棉兰皱皮螨、害嗜鳞螨亲缘关系比较近。磷酸化位点预测显示：Derf21含有1个丝氨酸激酶、1个苏氨酸激酶及1个酪氨酸激酶。二级结构及三级结构预测结果显示Derf21的结构主要以a螺旋为主。B细胞抗原表位预测得到5个肽序列（23.28、39.46、55.71、91.99、127.133)。丁细胞抗原表位预测得到4个肽序列（8.17、72.80、83.91和118.126)。结论对粉尘螨过敏原Derf21的分子特征进行预测，为进一步研究尘螨过敏原的结构成分及其理化性质奠定理论基础。Objective To analyze the characteristics of dust mite allergen Der f21 using a bioinformatics approach. Methods The clustalw2,MEGA5,ProtParam,KinasePhos,PSIPRED,SWISS-MODEL and DNAStar were used for homology analysis, phylogenetic tree construction,physicochemical property prediction, phosphorylation site prediction, secondary structure prediction,tertiary structure prediction and B cell epitope prediction,respectively. In addition, IEDB,Preprod, SYFPEITHI, NetMHCII 2. 2 Server and NetMHCII pan 3. 0 Sever were used for the prediction of T cell epitope of Der f21 protein. Results The gene sequence of Der f21 cloned in our laboratory shared 71 % homology with that published in GenBank(GenBank: ABC73706. 1).Phylogenetic tree analysis showed that Dermatophagoides farinae Der f21 had a close relationship with Dermatop hagoides pteronyssinus, Suidasia medanensis and Lepidoglyphus destructor. Phosphorylation site prediction showed that Der f21 contained 1 serine kinase, 1 threonine kinase and 1 tyrosine kinase. Secondary and tertiary structure prediction showed that Der f21 mainly consisted of a-helices. Bioinformatics analysis predicted 5 peptides(23-28,39-46,55-71,91-99 and 127-133) as B cell epitopes and 4 peptides(8-17 ? 72-80 ? 83-91 and 118-126) as T cell epitopes. Conclusion The prediction of molecular characteristics of dust mite allergen Der f21 lays a theoretical foundation for further study of compositions and physicochemical properties of dust mite allergens.

关 键 词：粉尘螨 Derf21 生物信息学分析 

分 类 号：R392.8[医药卫生—免疫学]