生姜提取物6-姜酚对毛发生长的影响和机制探讨  

Study on the mechanism and application of 6-Gingerol in regulating hair cycle

作　　者：侯春[1] 苗勇[2] 冀航[1] 王肃生[1] 梁刚[1] 张志华[1] 洪伟晋 HOU Chun;MIAO Yong;JI Hang;WANG Su-sheng;LIANG Gang;ZHANG Zhi-hua;HONG Wei-jin(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,Guangdong,China;South Hospital of Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]广州医科大学附属第一医院,广东广州510120 [2]南方医科大学南方医院,广东广州510515

出　　处：《中国美容医学》2016年第11期58-60,共3页Chinese Journal of Aesthetic Medicine

基　　金：2016年广州市中医药和中西医结合科技项目(20162A011017)

摘　　要：目的:探讨生姜提取物6-姜酚对毛发生长的影响和机制研究。方法:取高、中、低不同浓度6-姜酚分别与C57BL/6小鼠触须毛囊一起培养,11d后采用MTT法测定不同浓度6-姜酚与DPCs共培养的吸光度A值;比较不同浓度6-姜酚涂抹小鼠背部脱毛区皮肤后毛发的生长长度和皮肤组织中MMP-2,MMP-9 m RNA的数值变化。结果:不同浓度6-姜酚对DPCs以及DPCs中表达活性影响结果不同,结果表明高剂量组吸光度值明显小于低剂量组;在体内实验中,高剂量组C57BL/6小鼠背部毛发生长长度明显低于中、低剂量组,MMP-2,MMP-9 m RNA的表达随时间增加。结论:6-姜酚可明显抑制毛发的生长,其作用机制可能为通过增加皮肤组织中MMP-2,MMP-9 m RNA的表达量抑制毛发的生长。Objective To investigate the mechanism and application of 6-gingerol in the growth of hair,the main active component of ginger. Methods It was taken high,medium and low different concentration of 6-gingerol and C57BL/6 mouse vibrissa follicles cultured together.After 11 days,MTT method was used to measure the different concentration of 6-gingerol and DPCs co culture of absorbed value; comparison of different concentration of 6-gingerol smear depilating area of the back skin of mice after,hair growth length and MMP-2,MMP-9 mRNA expression. Results Different concentration of 6-gingerol of DPCs and DPCs expression of activity were different.The results showed that the high dose group of absorbance value was significantly lower than that in low dose group. In the in vivo experiment,the hair growth length of C57BL/6 mice in high dose group was significantly longer than that in middle and low dose group,the expression of MMP-2,MMP-9 mRNA increased as time goes. Conclusion 6-Gingerol can suppress human hair shaft elongation.The mechanism of action is to inhibit the growth of hair increasing the expression of MMP-2,MMP-9 mRNA.

关 键 词：细胞凋亡 6-姜酚 毛发生长机制 基质金属蛋白酶 毛乳头细胞 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]