盐酸氯苯胍在红笛鲷体内的药代动力学及残留消除规律  

Pharmacokinetics and Elimination Regularity of Robenidine Hydrochloride Residues in Luthjanus sanguineus

作　　者：汤菊芬[1,2] 蔡佳[1,2] 黄瑜[1,2] 廖建萌 黄月雄 简纪常[1,2] TANG Ju-fen;CAI-jia;HUANG Yu;LIAO Jian-meng;HUANG Yue-xiong;JIAN Ji-chang(Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China;Zhanjiang Food and Drug Inspection Institute, Zhanjiang 524022, China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088 [3]湛江市食品药品检测所,广东湛江524022

出　　处：《广东海洋大学学报》2016年第6期33-37,共5页Journal of Guangdong Ocean University

基　　金：农业部公益性行业专项(20120385);广东省科技计划(农业攻关)项目(2012B020308010)

摘　　要：在水温(28±2)℃、盐度28条件下,将盐酸氯苯胍(Robenidine hydrochloride,ROBH)按30 mg/kg的剂量口灌红笛鲷(Luthjanus sanguineus),用HPLC-MS/MS法研究ROBH在红笛鲷体内的药代动力学和残留消除规律。结果表明:单剂量口灌给药后,红笛鲷血浆中ROBH的药时数据符合一级吸收二室模型,药物在血浆中的达峰时间t_p为1.79 h,血药浓度峰值C_(max)为211.38μg/L,药时曲线下面积(AUC_(0-∞))为5 229.16μg/(L·h),消除半衰期(t1/2β)为46.99 h。ROBH在红笛鲷其他组织中分布较广,消除速度较慢,肌肉、肝脏和肾脏的C_(max)分别为37.58、863.02、1 063.24μg/kg,t_p均为1.5 h,AUC0-∞分别1 017.03、20 841.20、30 743.04μg/(kg·h);t1/2β分别为26.35、14.05和24.31 h。连续5 d口灌给药后,红笛鲷血浆、肌肉、肝脏和肾脏中的药物消除半衰期t1/2分别为46.14、36.11、28.62、26.92 h。以10μg/kg为最高残留限量,肌肉作为食用靶组织,在本试验条件下,建议休药期不少于7 d。The pharmacokinetics and elimination regularity of Robenidine hydrochloride (ROBH) residueswere investigated in tissues of Luthjanus sanguineus with a 30 mg/kg body weight after oral administrationat the water temperature of (28±2) ℃ and salinity of 28 by HPLC-MS/MS method. The results showedthat the date of ROBH concentration-time in plasma of L. sanguineus could be described as atwo-compartment model with first-order absorption. The peak time (tp) was 1.79 h, and the maximumconcentration (Cmax) was 211.38 μg/L, the area under the concentration-time curve(AUC0-∞) was 5 229.16μg/(L·h), and the elimination hal-life (tl/2β) of plasma was 46.99 h. ROBH has a wide distribution inorganizations of L. sanguineus and eliminated slowly. The Cmax in muscle, liver and kidney were 37.58,863.02 and 1 063.24 μg/kg; tp were all 1.50 h; AUC0-∞ were 1 017.03, 20 841.20 and 30 743.04 μg/(kg·h);tl/2β were 26.35, 14.05 and 24.31 h, respectively. After oral administration continuous with 30 mg/kg dosefor 5 days, the t 1/2 in plasma, muscle, liver and kidney of L. sanguineus were 46.14, 36.11, 28.62, and26.92 h, respectively. If the maximum residue limits (MRL) is 0.01 mg/kg and the muscles as the edible target tissue, the withdraw period should not be less than 7 days under this experiment condition.

关 键 词：盐酸氯苯胍 红笛鲷 药代动力学 药物残留 消除 

分 类 号：S943.231[农业科学—水产养殖]