低盐胁迫下红鳍东方鲀幼鱼鳃、肾和肠组织结构的变化  

Structural Changes in Gill, Kidney and Intestine of Juvenile Takifugu rubripes under Low Salinity Treatment

作　　者：孙梦蕾 蒋洁兰[1] 王莉苹[1] 陈飞[1] 韩雨哲[1] 姜志强[1] 暴宁 司滨 SUN Meng-lei;JIANG Jie-lan;WANG Li-ping;CHEN Fei;HAN Yu-zhe;JIANG Zhi-qiang;BAO Ning;SI Bin(Key laboratory of fish applied biology and aquaculture in North China, Liaoning Province; Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, China,Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学辽宁省北方鱼类应用生物学与增养殖重点实验室,农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《广东海洋大学学报》2016年第6期38-43,共6页Journal of Guangdong Ocean University

基　　金：辽宁省科技厅重大项目(2014203015);辽宁省教育厅一般项目(L2015076);大连海洋大学引进人才启动项目(HDYJ201501);农业部北方海水增养殖重点实验室开放课题(2015-MSENC-KF-04)

摘　　要：对低盐胁迫下体质量(11.34±2.13)g红鳍东方鲀(Takifugu rubripes)的鳃、肾和肠组织结构进行显微观察,探讨组织结构变化与渗透压的关系。结果表明,低盐胁迫下,鳃丝和鳃小片宽度增大,鳃小片间距缩小,与对照组差异均有统计学意义(P<0.05),鳃小片细胞更为圆润饱满,低盐度组每100μm泌氯细胞数为0.91±0.78,低于对照组,差异有统计学意义(P<0.05);低盐度组的肾小球膨大,饱满充盈,与肾小囊内壁间隙小于对照组,差异有统计学意义(P<0.05),近曲小管和集合管管径增大,近曲小管游离面刷状缘较对照组发达;低盐度组肠绒毛单层柱状上皮厚度为(51.54±2.53)μm,大于对照组(P<0.05),柱状上皮细胞间每100μm杯状细胞数为5.05±0.91,小于对照组(P<0.05),而两组间的杯状细胞大小差异无统计学意义(P>0.05)。水体盐度变化导致红鳍东方鲀鳃、肾和肠发生适应性变化。Structural changes in gill, kidney and intestine of juvenile Takifugu rubripes with bodyweight of 11.34 ± 2.13 g were observed under low salinity treatment by using the histologicalphotomicrography technique. The relationships between structural changes and osmotic regulation werediscussed. The results showed that the width of gill filaments and gill lamellae increased and the spaceof adjacent gill filaments reduced under low salinity condition with the statistical difference from thecontrol (P < 0.05). The gill lamellae cells became plumper in the experimental group. The number ofchloride cells was 0.91±0.78 cells per 100 μm, which was obviously lower than that of the control group(P < 0.05). Compared with the control group, the glomerular expanded and the gap between theglomerular and the inner wall of the renal capsule became significantly smaller (P < 0.05). In the lowsalinity group, thickness of simple columnar epithelium was 51.54 ± 2.53 μm, remarkably higher than that in the control group (P < 0.05). The number of goblet cells (5.05 ± 0.91 cells per 100 μm) amongcolumnar epithelial cells decreased significantly (P < 0.05) while the body size of goblet cell had nosignificant difference within two groups (P > 0.05). The findings indicated that the variety of salinitycould lead to adaptive changes in gill, kidney and intestine of juvenile Takifugu rubripes. These changeswere beneficial to enhance environmental adaptability of Takifugu rubripes. This research could providea theoretical basis for further study in osmitic regulation mechanism of Takifugu rubripes.

关 键 词：红鳍东方鲀 鳃 肾 肠 结构 低盐胁迫 

分 类 号：Q954.5[生物学—动物学]