地佐辛预先给药对大鼠中脑导水管周围灰质c-fos表达的影响  

EFFECTS OF DEZOCINE PRETREATMENT ON EXPRESSION OF C-fos PROTEIN IN PERIAQUEDUCTAL GRAY OF RATS WITH OF INCISIONAL PAIN

作　　者：吕志峰[1] 方洁[1] 朱建坡[1] 张虎[1] 李阿丽[1] 程志芳[1] 任学军[1] 吕帅国[2] LV Zhi-Feng;FANG-Jie;ZHU Jian-Po;ZHANG-Hu;LI A-Li;CHENG Zhi-Fang;REN Xue-Jun;LV Shuai-Guo(1Department of Anesthesiology, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, 450000, China;Department of Anesthesiology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, 450000, China)

机构地区：[1]河南中医学院第一附属医院麻醉科,郑州450000 [2]河南省肿瘤医院麻醉科,郑州450000

出　　处：《中国疼痛医学杂志》2016年第12期896-899,共4页Chinese Journal of Pain Medicine

摘　　要：目的:观察地佐辛预先给药对切口痛大鼠中脑导水管周围灰质c-fos蛋白表达的影响。方法:成年雄性Wistar大鼠,36只,随机分为3组(n=12),对照组(C组)仅给予麻醉而不手术;生理盐水组(S组):切口疼痛模型手术前15 min尾静脉注射0.9%氯化钠溶液2 ml;地佐辛组(D组):切口疼痛模型手术前15 min尾静脉注射地佐辛3 mg/kg 2 ml。于术前24 h(T_0)、术后2 h、6 h、24 h(T_(1-3))时测定机械缩足反射阈值(mechanical withdrawal threshold,MWT)和累积疼痛评分,T3时痛阈测定后处死大鼠,取大鼠全脑,采用免疫组化方法检测中脑导水管周围灰质神经元c-fos蛋白表达。结果:与C组相比较,S组和D组T1-3时MWT明显降低,累积疼痛评分升高,中脑导水管周围灰质神经元c-fos蛋白免疫阳性细胞数增高(P<0.05);与S组比较,D组T1-3时MWT升高,累积疼痛评分降低,中脑导水管周围灰质神经元c-fos蛋白免疫阳性细胞数下降(P<0.01)。结论:地佐辛预先给药可抑制中脑导水管周围灰质神经元c-fos蛋白表达,减轻大鼠切口痛。Objective: To observe the effect of dezocine pretreatment on c-fos protein expression in periaqueductal gray of rats with incisional pain. Methods: Thirty-six male Wistar rats were randomly divided into 3 groups (n =12 each): control group(group C), normal saline group (group S), and dezocine group (group D).In the saline or dezocine group, rats were injected with normal saline 2 ml or dezocine 3 mg/kg (in 2 ml of volume) via tail vein. Rats in group S and group D received surgery to establish incisional pain model 15 min after tail vein injection. The mechanical paw withdrawal threshold (MWT) and cumulative pain score were measured 24 h before surgery (T0, baseline) and 2 h, 6 h, 24 h after surgery (T1-3). The rats were sacrificed after all of the pain threshold measurement and the whole brains were collected for detecting the c-fos expression in periaqueductal gray by immunohistochemistry assay. Results: In group S and group D, the MWTs were significantly decreased, cumulative pain scores were significantly increased, and the expression of c-fos protein was significantly up-regulated at T1-3, compared with group C. Compared with group S,the rats in group D exhibited increased MWT, decreased cumulative pain score, and down-regulated c-fos expression at T1-3. Conclusion: Dezocine can inhibit the up-regulation of c-fos protein expression and thus attenuate the incisional pain in rats.

关 键 词：地佐辛 预先给药 切口痛 中脑导水管周围灰质 C-FOS蛋白 

分 类 号：R614[医药卫生—麻醉学]