温郁金挥发油通过PI3K/Akt信号途径对阿尔茨海默模型小鼠tau蛋白磷酸化表达的影响  

Effects of Wenyujin Essential Oil on tau Protein Phosphorylation in Mice with Aβ-induced Alzheimer Disease through PI3k/Akt Pathway

作　　者：齐越[1] 秦文艳[1] 康凯[2] 姜鸿[1] 李昭[2] 王亚斌[2] 贾冬[2] QI Yue;QIN Wen-yan;KANG Kai;JIANG Hong;LI Zhao;WANG Ya-bin;JIA Dong(Department of Pharmacology, The Second Hospital Affiliated to Liaoning Chinese Medical University, Shenyang 110034, China;Laboratory Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China)

机构地区：[1]辽宁中医药大学附属第二医院,辽宁沈阳110034 [2]辽宁中医药大学实验中心,辽宁沈阳110847

出　　处：《中国中医药信息杂志》2017年第1期45-48,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技重大专项-重大新药创制(2012ZX09102201-005)

摘　　要：目的观察温郁金挥发油对阿尔茨海默病(AD)模型小鼠tau蛋白磷酸化的影响,探讨其相关作用机制。方法 60只昆明种小鼠随机分为假手术组、模型组、温郁金挥发油低剂量组、温郁金挥发油高剂量组、盐酸多奈哌齐组和参枝苓组,海马组织注射Aβ25-35制作AD小鼠模型,各给药组给予相应药物灌胃,连续15 d。末次给药后,免疫组化检测小鼠tau蛋白磷酸化位点(Ser 404和Thr 231)的蛋白表达,Western blot检测tau蛋白磷酸化位点及磷脂酰肌醇-3-激酶信号/蛋白激酶B(PI3K/Akt)的蛋白表达。结果与假手术组比较,模型组tau蛋白磷酸化位点蛋白表达增加(P<0.05),PI3K及Akt磷酸化水平明显降低(P<0.05);与模型组比较,温郁金挥发油高剂量组tau蛋白(Thr231和Ser404)磷酸化水平明显降低(P<0.05),PI3K及Akt磷酸化水平明显增加(P<0.05,P<0.01)。结论温郁金挥发油可降低模型小鼠tau蛋白磷酸化水平,其作用机制可能与PI3K/Akt信号途径有关。Objective To investigate the effects of Wenyujin essential oil on tau protein phosphorylation in mice with Alzheimer Disease (AD); To discuss the relevant mechanism of action. Methods Sixty Kunming mice were divided into six groups randomly: sham-operation group, model group, Wenyujin essential oil low- and high-dose group, donepezil group and Shenzhiling group. β-Amyloid protein (Aβ25-35) was infused into the hippocampal of mice.Mice in each group were given relevant medicine for gavage for 15 d. After the last administration,immunohistochemistry was used to detect the expressions of tau protein phosphorylation points (Ser 404 and Thr 231). Western blot was used to detect tau protein phosphorylation points and the expression of PI3K/Akt protein.Results Compared with sham-operation group, tau protein phosphorylation points in the model group increased (P<0.05), but the phosphorylation levels of PI3K and Akt decreased significantly (P<0.05). Compared with the model group, tau protein phosphorylation (Thr231 and Ser404) in Wenyujin essential oil high-dose group decreased significantly (P<0.05), and the phosphorylation levels of PI3K and Akt increased (P<0.01).Conclusion Wenyujin essential oil can inhibit tau protein phosphorylation in mice. The possible mechanism may be related to the PI3K/Akt signal pathway.

关 键 词：温郁金挥发油 阿尔茨海默病 TAU蛋白 磷脂酰肌醇-3-激酶信号/蛋白激酶B 小鼠 

分 类 号：R285.5[医药卫生—中药学]