补肾活血方对老年大鼠心脏自由基代谢及p16蛋白表达的影响  

Effects of Bushen Huoxue Formula on Free Radical Metabolism and p16 Protein Expression in Heart of Aged Rats

作　　者：陈靓[1] 崔开宇[1] 吴勇[1] 杨伟峰[2] CHEN Liang;CUI Kai-yu;WU Yong;YANG Wei-feng(Hubei University of Chinese Medicine, Wuhan 430065, China;Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]中国中医科学院医学实验中心,北京100700

出　　处：《中国中医药信息杂志》2017年第1期55-58,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖北省自然科学基金(2012FKB02102)

摘　　要：目的观察补肾活血方对老年大鼠心脏自由基代谢和p16蛋白表达的影响,探讨其对衰老大鼠心脏的保护作用机制。方法 100只大鼠随机分为青年对照组、老年对照组和补肾活血方高、中、低剂量组,每组20只。各给药组给予相应药物灌胃,每日1次,连续16周。末次给药后1 h,将大鼠麻醉后取血清和心脏。测定血清和心肌组织一氧化氮(NO)、丙二醛(MDA)含量和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性;免疫组化检测心肌组织β-半乳糖苷酶和p16蛋白表达。结果与老年对照组比较,补肾活血方高剂量组大鼠血清NO含量和CAT、SOD活性显著升高(P<0.05),补肾活血方各剂量组MDA含量显著降低(P<0.01);补肾活血方高剂量组大鼠心肌组织NO含量和SOD活性显著升高,MDA含量显著降低(P<0.05),补肾活血方各剂量组CAT活性显著升高(P<0.05,P<0.01);补肾活血方各剂量组心肌组织β-半乳糖苷酶和p16蛋白表达降低。结论补肾活血方通过抗氧化损伤和抑制抑癌基因p16表达,发挥抑制老年大鼠心肌老化的作用。Objective To observe the effects of Bushen Huoxue Formula on free radical metabolism and p16 protein expression in heart of aged rats; To discuss its protective mechanism to the heart of aged rats. Methods One hundred were divided into young control group, the natural aging group, Bushen Huoxue Formula high-, medium- and low-dose groups, with 20 rats in each group. Each medication group was given relevant medicine for gavage, once a day for 16 weeks. 1 hour after the last administration, after the rats were sacrificed, serum and heart were taken. The contents of NO and MDA and activities of CAT and SOD in serum and myocardial, β-galactosidase enzyme and p16 protein expression in myocardial tissue were detected. Results Compared with the natural aging group, NO content and SOD activity in the serum of rats in Bushen Huoxue Formula high-dose group increased significantly (P<0.05) and MDA content in all Bushen Huoxue Formula groups decreased (P<0.01); NO content, CAT and SOD activity in the myocardial tissue of rats in Bushen Huoxue Formula high-dose group increased significantly, and MDA content decreased significantly (P<0.05). CAT activity in all Bushen Huoxue Formula groups increased (P<0.05, P<0.01).β-galactosidase enzyme and p16 protein expression in myocardial tissue in all Bushen Huoxue Formula groups decreased. Conclusion Bushen Huoxue Formula can in hibit the aging of myocardial-aged rats, and the mechanism might be related to its anti-oxidative damage and inhibition of tumor suppressor gene p16 expression.

关 键 词：补肾活血方 衰老大鼠 氧化损伤 P16蛋白表达 

分 类 号：R285.5[医药卫生—中药学]