低剂量胞外组蛋白对内皮细胞毒性研究  

Study of endothelial cell toxicity of low dosage of extracellular histones

作　　者：朱明昂 陶悦[1] 曹清 周云芳[2] 莫茜[1] ZHU Mingang;TAO Yue;CAO Qing;ZHOU Yunfang;MO Xi(Key Laboratory of Pediatric Hematology & Oncology Ministry of Health, Pediatric Translational Medicine Institute Shanghai Children’s Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200127 , China;Department of Infections, Shanghai Children’s Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200127 , China)

机构地区：[1]上海交通大学医学院附属上海儿童医学中心卫生部儿童血液肿瘤重点实验室儿科转化医学研究所,上海200127 [2]上海交通大学医学院附属上海儿童医学中心感染科,上海200127

出　　处：《临床儿科杂志》2016年第12期942-948,共7页Journal of Clinical Pediatrics

基　　金：国家自然科学青年基金(No.81201450);上海市科委启明星计划(No.14QA1402900);上海市自然科学基金(No.16ZR1421600)

摘　　要：目的探讨低剂量组蛋白在脓毒症等感染性疾病中对内皮细胞的作用特征。方法以实验室体外表达纯化的重组人源化组蛋白H3/H4复合物纯品代替小牛胸腺组蛋白,对内皮细胞进行低剂量(10μg/m L)、长时间(48 h)处理。观察细胞形态和活性,流式细胞术检测活细胞百分比,检测细胞上清组蛋白含量。结果低剂量组蛋白长时间处理可引起内皮细胞发生持续的细胞死亡,细胞形态变化和功能丧失。这一毒性作用在10μg/m L组蛋白H3/H4处理时得到重复,且定量结果显示其在短时间内即可作用于细胞内部引起一系列胞内反应。结论比以往实验处理条件更低和更接近于病理情况的10μg/m L的H3/H4就能有效引起脓毒症等重症感染性疾病的体内毒性作用。Objective To investigate the effects of low dose of extracellular histone on endothelial cells in infectious diseases such as sepsis. Methods The endothelial cells were treated with 10 μg/mL recombinant human histone H 3 /H 4 complex in replacement of calf thymus histones (CTH) for various periods of time, and the morphology changes and the viability of the endothelialcells were recorded. In addition, flow cytometry was applied to identify the characteristics of endothelial cells and enzyme-linked immunosorbnent assay (ELISA) was used to detect the extracellular histones level in endothelial cells culture. Results The low doseof CTH could continuously induce endothelial cells death, cell morphological changes and function loss, which was reproduced by 10μg/mL recombinant histone H3/H4 complex. Results of histones quantitation showed that histone can cause a series of intracellularreactions in a short period of time. Conclusions It is showed that 10 μg/mL H3/H4 can induce the toxicity in infectious disease and this level of the dose is a lower than those used in previous studies and more close to the pathological conditions.

关 键 词：胞外组蛋白 内皮细胞 细胞毒性 脓毒症 

分 类 号：R459.7[医药卫生—急诊医学]