一株脱硫菌株的分离鉴定及其对硫化物的去除效果验证  

Isolation and Identification of a Desulfurization Strain and Its Sulfide Removal Performance

作　　者：赵鹏[1,2] 李东[1] 周一民[3] 刘晓风[1] 廖银章[1] ZHAO Peng;LI Dong;ZHOU Yi-min;LIU Xiao-feng;LIAO Yin-zhang(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;University of Chinese Academy of Sciences,Beijing 100049, China;Chengdu Organic Chemicals Co., Ltd., Chinese Academy of Sciences, Chengdu 610041, China)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]中国科学院成都有机化学有限公司,成都610041

出　　处：《新能源进展》2016年第6期425-430,共6页Advances in New and Renewable Energy

基　　金：国家科技支撑计划项目(2015BAD21B01)

摘　　要：为获得适合生物脱硫工程应用的硫氧化细菌，取污水处理厂曝气池活性污泥，采用人工模拟硫化物废水作为硫源富集培养并经分离纯化，得到一株能够高效快速去除废水中硫化物的硫氧化细菌菌株DS-7。该菌为短杆状、长0.8~2μm、宽0.6~0.8μm、革兰氏阴性，最适生长温度为30℃，最适生长pH值为7.0。通过对该菌的16SrDNA序列测定，并结合其形态和生理特性鉴定，该菌株属于鞘氨醇杆菌属（Sphingobacterium）。Sphingobacteriumsp.DS-7去除硫化物性能研究结果表明：当硫化物浓度为650mg/L时，该菌的4h硫化物去除率达到95%以上，相应的硫转化率最高达到45.08%，生成的单质硫颗粒形状为不规则八面体。In order to obtain sulfur-oxidizing bacteria for engineering application of biodesulfurization, the enrichment andacclimation of aerobic activated sludge was carried out by using artificial sulfide wastewater as a sulfur source. Asulfur-oxidizing bacteria strain DS-7 with strong ability of sulfide removal was isolated from the enrichment culture. Thestrain DS-7 was Gram-negative and short rod shaped with length of 0.8 ~ 2 μm and width of 0.6 ~ 0.8 μm. The optimalgrowth temperature and pH was 30oC and 7.0, respectively. The identified strain DS-7 belongs to the genus ofSphingobacterium according to the 16SrDNA bacterial sequences and the morphological and physiological characterization.The sulfide removal performance tests of Sphingobacterium sp. DS-7 showed that the sulfide removal rate was more than95% and the sulfur conversion rate reached 45.08% after culturing for 4 h at the initial sulfide concentration of 650 mg/L.The sulfur formed by Sphingobacterium sp. DS-7 was in the shape of irregular octahedron.

关 键 词：硫氧化细菌 生物脱硫 硫化物 16SrDNA 鉴定 

分 类 号：TK6[动力工程及工程热物理—生物能]