121份菜豆品种资源的植物凝集素含量分析  

Lectin Content Analysis of 121 Common Bean Cultivars

作　　者：任三国 陈高[1] 郭瑞[1] 陈禅友[1] REN Sanguo;CHEN Gao;GUO Rui;CHEN Chanyou(School of Life Sciences,Jianghan University;Huhei Province Engineering Research Center for Legume Plants,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学生命科学学院,湖北省豆类(蔬菜)植物工程技术研究中心,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2016年第6期504-508,共5页Journal of Jianghan University：Natural Science Edition

基　　金：农业部948项目(2011-G1-17);湖北省科技平台项目(鄂科技通[2011]第101号);湖北省资源中心项目(2015BCE091);武汉市科技攻关项目(201250499145-11)

摘　　要：菜豆鲜荚中含有多种抗营养因子，其中菜豆凝集素为最主要的毒性因子，摄入不当会导致食物中毒。以121个菜豆品种商品荚为试验材料，测定其植物凝集素含量，分析评价其遗传变异，扩大极低和极高菜豆凝集素水平的筛查范围。结果表明：121个菜豆品种的凝集素含量水平差异极显著（P<0.01），变异系数为98.34％。121个菜豆品种植物凝集素含量变幅为0.005～1.330mg/g，均值为0.412mg/g，品种频率分布曲线主峰明显，呈近似正态分布，但是两尾偏长，说明有极低和极高菜豆凝集素含量的品种分布。筛选出低菜豆植物凝集素的品种3份，为低毒菜豆遗传改良和合理利用提供技术支持。Fresh pods of common bean contain a variety of anti-nutritional factors,lectin is one of the main endogenous toxins. It can cause poisoning phenomena while improper eating. In this study,the contents of lectin in fresh pods were detected in 121 selected common bean cultivars,the geneticvariations were analysed,the screening range was enlarged with extreme low and extreme high lectincontent. The results revealed significant difference of lectin in the tested cultivar population(P < 0. 01),its coefficients of variation( CV )was 98. 34%. Meanwhile,contents of lectin in all cultivars rangedfrom 0. 005 mg/g to 1. 330 mg/g,and the mean value was 0. 412 mg/g. The cultivar frequencydistribution curve showed a main peak and nearly normal distribution,but slightly long on dual-tails,which demonstrated the distribution of cultivars with extreme low or high level on lectin. Therefore,three cultivars containing less lectin were identified. It will provide experimental basis and data support for genetic improvement and rational utilization of non endogenous toxicity germplasm.

关 键 词：菜豆 品种 植物凝集素 

分 类 号：S643.1[农业科学—蔬菜学]