光催化合成银纳米簇对半胱氨酸的检测  

Photocatalytic Synthesis of Silver Nanoclusters for Detection of Cysteine

作　　者：周林宗[1] 王干珍[2] 易晓明[2] 朱丽琴[2] 彭君[2] ZHOU Lin-zong;WANG Gan-zhen;YI Xiao-ming;ZHU Li-qin;PENG Jun(The Ministry of Land and Resources of Changsha Mineral Resources Supervision and Inspection Center,Changsha, Hunan, 410007, China;Chuxiong Normal University, Chuxiong, Yunnan, 675000, China)

机构地区：[1]楚雄师范学院,云南楚雄675000 [2]国土资源部长沙矿产资源监督检测中心,湖南长沙410007

出　　处：《工业技术创新》2016年第6期1060-1064,共5页Industrial Technology Innovation

摘　　要：以木瓜蛋白酶为模板,通过光催化法合成了光学性质稳定、生物相容性好的银纳簇。研究发现,将一定浓度的半胱氨酸加入银纳米簇和银离子的混合溶液后,混合溶液在365 nm处会出现一个等离子共振吸收峰,且与半胱氨酸的浓度有良好的线性关系。因此,建立了半胱氨酸的紫外吸收光谱分析法,实现了0.5μM^60.0μM浓度范围内半胱氨酸的定量测定,检出限达0.5μM。这种检测方法具有良好选择性,常见的生物小分子均不会对检测造成影响。Silver nanoclusters(AgNCs)with excellent photostability and biocompatibility have beensynthesized using photocatalysis method in which papain is used as a template.When cysteine(Cys)withcertain concentration is added into mixed solution of AgNCs and silver ions,a strong absorption band near365nm could be found in their absorption spectra owing to localized surface plasmon resonance(LSPR)ofproduced AgNCs.The absorbance changes at365nm have a good relationship with Cys concentration.Thepresent assay allows for the sensing of Cys in the range from0.5μM to60.0μM,with a limit of detection(LOD)as low as0.5μM.More importantly,this colorimetric method has high selectivity to Cys.Potentialinterferes,such as glucose and dopamine will not affect the detection of Cys.

关 键 词：银纳米簇 光催化 紫外分光光度法 半胱氨酸 

分 类 号：O6[理学—化学]