Meox1在心肌梗死大鼠心肌中的表达情况  

Changes in the expression of Meox1 after acute myocardial infarction in rats

作　　者：沈俊[1,2] 袁平[2] 唐俊明[1] 杨建业[1] 李兴元[1] 张蕾[1] 赵继先[2] 张焕鑫[2] 薛仕珍[2] 冯怡[2] 王家宁[1,2] SHEN Jun;YUAN Ping;Tang Jun-ming;YANG Jian-ye;LI Xing-yuan;Zhang Lei;ZHAO Ji-xian;ZHANG Huan-xin;XUE Shi-zhen;FENG Yi;WANG Jia-ning(Institute of Clinical Medicine and Department o f Cardiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China)

机构地区：[1]湖北医药学院附属人民医院临床医学研究所,十堰442000 [2]湖北医药学院附属人民医院心脏病中心1病区,十堰442000

出　　处：《中国循证心血管医学杂志》2016年第11期1329-1332,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

基　　金：国家自然科学基金(81270221)

摘　　要：目的分析同源盒基因Meox1在心肌梗死大鼠心肌中的表达情况。方法清洁级雄性SD大鼠20只,随机分入心肌梗死组和假手术组,每组10只。心肌梗死组采用结扎左冠状动脉前降支构建心肌梗死模型,手术过程死亡2只。假手术组仅穿线不结扎。术后7 d处死大鼠,制作石蜡切片,HE染色以及Masson染色观察心肌组织变化,免疫组化法检测Meox1的表达水平。结果 HE染色结果:心肌梗死组可见梗死区心肌细胞显著减少,核碎裂,核消失,被大量排列紊乱的结缔组织代替,可见大量粒细胞、单核细胞浸润。梗死边缘区可见心肌细胞代偿性肥大,横纹模糊或消失,心肌间隙水肿增宽,部分心肌纤维溶解、断裂,可见炎性细胞浸润、成纤维细胞增生。梗死远离区及假手术组大鼠心肌形态正常,结构清晰,心肌纤维排列整齐。Masson染色结果,非梗死区染色呈红色为正常心肌组织,蓝色为胶原纤维。假手术组及梗死远离区心肌细胞排列整齐紧密,细胞间隙散在分布少量胶原纤维;心肌梗死组梗死区可见细胞间隙大量胶原纤维沉积,梗死边缘区的细胞间隙也有大量的胶原纤维沉积。免疫组化结果,梗死区及梗死边缘区的心肌细胞中可见棕黄色颗粒沉积,有Meox1表达;在梗死远离区的正常心肌细胞中未见Meox1的表达;假手术组心肌细胞中亦未见Meox1的表达。假手术组及梗死远离区毛细血管内皮细胞中未见Meox1的表达;心肌梗死组梗死区及梗死边缘区毛细血管内皮细胞中Mexo1表达不明显。结论在心肌梗死大鼠心肌组织中Meox1表达增高,提示其可能参与心肌梗死后心肌重塑。Objective To analyze the expression of Meox1in myocardium in rats with acute myocardial infarction(AMI).Methods Male SD rats(n=20)were randomly divided into AMI group and sham-operation group(each n=10).Themodel of AMI was established by ligation of left anterior descending branch of coronary artery in AMI group(2rats diedduring the procedure),and sham-operation group was given the same procedure except of ligation.The rats were killed7d after the procedure for preparing para^n sections,and changes of myocardial tissue were observed after HE staining andMasson staining and expression of Meox1was detected by using immunohistochemistry technique.Results The resultsof HE staining showed that cardiomyocyte decreased significantly at infarction zone,and there were karyorrhexis,nucleardisappearing,disorganized connective tissue in quantity,and severe infiltration of granulocytes and monocytes in AMIgroup.The compensatory hypertrophy and cross striatation blurring or disappearing of cardiomyocytes,widened myocardialgap edema,fibrinolysis and fragmentation of partial myocardial fibers,infiltration of inflammatory cells and proliferation offibroblast,were observed at infarction marginal zone.The normal myocardial histology,clear structure and aligned myocardialfibers were observed at infarction remote zone and in sham-operation group.The results of Masson staining showed that redwas normal myocardial tissue and blue was collagen fibers at non-infarction zone.The cardiomyocytes were aligned andtight,and there were less collagen fibers in intercellular space in sham-operation group and at infarction remote zone.Therewas deposition of collagen fibers in quantity in intercellular space and at infarction zone and infarction marginal zone inAMI group.The results of immunohistochemistry technique showed that there was deposition of brown granules and Meox1expression in cardiomyocytes at infarction zone and infarction marginal zone,and there was no Meox1expression in normalcardiomyocytes at infarction remote zone and in sham-oper

关 键 词：心肌梗死 同源盒基因Meox1 表达 

分 类 号：R541.4[医药卫生—心血管疾病]