一株溶藻细菌的分离、筛选与分子鉴定  

Isolation, screening and molecular identification of an algae-lying bacterium

作　　者：朱晓漫 罗玉双[1,2,3] 李娜[1,2,3] 邹万生[1,2,3] 熊鹏[1,2,3] 潘慧[1,2,3] 李红丽[1,2,3] Zhu Xiaoman;Luo Yushuang;Zou Wansheng;Xiong Peng;Pan Hui;Li Hongli(Collaborative Innovation Center for Efficient and Health Production in Hunan Province, Hunan University of Arts and Science, Changde 415000, China;Key Laboratory of Health Aquaculture and Product Processing in Dongting Lake Area of Hunan Province, Hunan University of Arts and Science, Changde 415000, China;Zoology Key Laboratory of Hunan Higher Education, Hunan University of Arts and Science, Changde 415000, China)

机构地区：[1]湖南文理学院水产高效健康生产湖南省协同创新中心,湖南常德415000 [2]湖南文理学院环洞庭湖水产健康养殖及加工湖南省重点实验室,湖南常德415000 [3]湖南文理学院动物学湖南省高校重点实验室,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2017年第1期28-34,共7页Journal of Hunan University of Arts and Science(Science and Technology)

基　　金：湖南省科技计划项目(No.2012FJ3091);湖南省高校创新平台开放基金项目(12K121);湖南省"十二五"重点建设学科(动物学);2015年湖南文理学院大学生创新创业研究项目(ZD1507);2015年湖南文理学院研究性教学课程项目

摘　　要：为了筛选对铜绿微囊藻有溶藻活性的菌株,采用试管法筛选溶藻效果好的细菌,显微观察高效溶藻菌的溶藻作用方式,对其热稳定性、耐酸碱能力及紫外稳定性进行考察,并采用16S r DNA序列分析鉴定溶藻菌种类。共筛选获得7株具有溶藻效果的细菌,其中F15菌株对铜绿微囊藻溶藻效果最好。菌株F15的溶藻活性物质为胞外产物,当其生长到对数期时,其胞外产物的溶藻效果达到最大,按体积比1∶6将其加入藻液,培养3 d后,铜绿微囊藻的去除率达55%以上。该溶藻活性产物对加热、酸碱及紫外线处理均具有较好的稳定性。16S r DNA序列分析表明,F15菌株与Bacillus cereus strain ATCC 14579同源性最高,达99%,被鉴定为蜡状芽孢杆菌。F15菌株溶藻效果好,稳定性高,具有开发成生物控藻菌剂的潜在价值。Isolation,screening and identification of algae-lying bacteria against Microcystis aeruginosa from bottomsediment of the fresh-water cultured bases.Bacteria with high algicidal activity against Microcystis aeruginosa werescreened by tube methods,the mode of algicidal action was observed by microscope,its thermal stability,acidalkaline and ultraviolet-resistant were assessed.The isolated algicidal bacterium was identified by16S rDNA genesequence analysis.Seven algae-lying bacteria were obtained,of these,F15strain has the best lying effect againstMicrocystis aeruginosa.The algae-lying substance of F15strain was extracellular products,it has the highest lyticefficiency at logarithmic growth station,more than55%of Microcystis aeruginosa had been removed by3daysafter adding the extracellular products with1:6volume ratio.It was stability to heat,pH and ultra-violet radiation.The16S rDNA sequence analysis of F15strain showed that it is homology with Bacillus cereus strain ATCC14579,the similarity was up to99%.F15strain was identified as Bacillus cereus.Taken together,F15strain has a greatalgicidal effect and a high stability,suggested that it has potential value for the development of biological agentagainst Microcystis aeruginosa.

关 键 词：铜绿微囊藻 溶藻细菌 分离与筛选 溶藻特征 分子鉴定 

分 类 号：Q959[生物学—动物学]