活血除痹汤对硬皮病小鼠皮肤血小板衍生生长因子A和转移生长因子-β表达的影响  

Effects of Huoxue Chubi Decoction on PDGF-A and TGF-β Expression in Skin of Scleroderma Mice Model

作　　者：潘厚儒[1] 陈曦[1] 张润田[1] 徐国梅[2] 段行武[1] PAN Hou-ru;CHEN Xi;ZHANG Run-tian;XU Guo-mei;DUAN Xing-wu(Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China;The Third Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学第三附属医院,北京100029

出　　处：《中国中医药信息杂志》2017年第3期48-52,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京中医药大学自主选题项目(2013-JYBZZ-XS-189)

摘　　要：目的观察活血除痹汤对硬皮病小鼠皮肤血小板衍生生长因子A(PDGF-A)和转化生长因子-β(TGF-β)表达的影响,探讨其可能的作用机制。方法 30只小鼠随机分为空白组、模型组和中药高、中、低剂量组,每组6只。除空白组外,其余各组小鼠均予皮内注射盐酸博来霉素0.1 m L,连续3周,建立硬皮病小鼠模型。第4~7周,空白组和模型组予等量蒸馏水灌胃,中药高、中、低剂量组分别给予活血除痹汤44.8、22.4、11.2 g/(kg·d)灌胃,每日1次。观察各组小鼠皮肤及肺组织病理学改变,HE染色测量皮肤厚度,免疫组化检测皮损组织PDGF-A、TGF-β和Ⅰ、Ⅲ型胶原(COL-Ⅰ/COL-Ⅲ)的蛋白表达。结果模型组小鼠真皮明显增厚,胶原纤维明显增粗,并有炎性细胞浸润;肺组织可见肺泡间隔增厚,有成纤维细胞形成并伴大量单核细胞浸润;与模型组比较,中药各剂量组皮肤厚度、真皮胶原纤维膨大增厚程度及肺泡间隔增粗程度均有所减轻。模型组PDGF-A、TGF-β、COL-Ⅰ及COL-Ⅲ蛋白表达均较空白组明显增加(P<0.01);中药各剂量组上述指标阳性表达均较模型组明显降低(P<0.01)。PDGF-A、TGF-β表达与COL-Ⅰ、COL-Ⅲ表达呈正相关。结论活血除痹汤可能通过抑制PDGF-A、TGF-β的表达,抑制胶原表达和成纤维细胞的生成,达到治疗硬皮病的作用。Objective To observe the effects of Huoxue Chubi Decoction on contents of PDGF-A and TGF-βinskin lesion of scleroderma mimicking mice models;To discuss its possible mechanism.Methods Thirty mice wererandomly assigned into blank group,model group,TCM high-,medium-,low-dose group,with six mice in eachgroup.Other than blank group,the rest of the4groups were given bleomycin-hydrocholoride0.1mL intradermalinjection daily for3weeks to establish scleroderma mice models.From week4to7,blank group and model groupreceived gavage with equal amount of distilled water daily;TCM high-,medium-,low dosage group received gavagewith Huoxue Chubi Decoction with different concentrations of44.8,22.4,11.2g/kg daily.The pathological changes inthe skin tissue samples taken from each group were observed under microscope;the thickness of skin from each groupwas measured and the expression of PDGF-A,TGF-β,type-Ⅰcollagen(COL-Ⅰ)and type-Ⅲcollagen(COL-Ⅲ)were tested through immunohistochemical staining.Results Compare with blank group,the dermis tissue of modelgroup was thicker,with the presence of thicker and greater number of collagen fibers,as well as infiltration ofinflammatory cells.Compare with model group,thickness of skin and dermis collagen growth of TCM high-,medium-,low-dose group were milder varied by the concentration of the decoction.The result ofimmunohistochemical staining showed that the expressions of PDGF-A,TGF-β,COL-Ⅰand COL-Ⅲof model group significantly increased than blank group(P<0.01);the expression of the mentioned indicators werestatistically significant lower in the TCM high-,medium-,low-dose group than model group;The expressions ofPDGF-A and TGF-βshowed a positive correlation with the amount of COL-Ⅰand COL-Ⅲpresented in eachgroup.Conclusion Huoxue Chubi Decoction can suppress collagen expression and fibroblast formation in skin lesionof scleroderma mice models by down regulating the expressions of TGF-βand PDGF-A,thus demonstrate itspotential in scleroderma treatment.

关 键 词：硬皮病 活血除痹汤 胶原蛋白 血小板衍生生长因子A 转化生长因子-Β 小鼠 

分 类 号：R285.5[医药卫生—中药学]