水体中蓝藻水华分解产甲烷动态过程研究  

Dissolved methane dynamics during the degradation of organic matter derived from cyanobacterial bloom

作　　者：胡万婷 唐千[2,3] 孙伟[2,4] 朱立峰[1] 邢鹏[2] HU Wan-ting;TANG Qian;SUN Wei;ZHU Li-feng;XING Peng(College of Life Sciences, Nanjing Normal University, Nanjing 210046, China;State Key Laboratory of Lake and Environment, Nanjing Institute of Geography Limnology, Chinese Academy of Sciences, Nanjing 210008, China;University of Chinese Academy of Sciences,Beijing 100039, China;Applied Meteorological Institute, Nanjing University of Information Science and Technology,Nanjing 210044, China)

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,江苏南京210008 [3]中国科学院大学,北京100039 [4]南京信息工程大学应用气象学院,江苏南京210044

出　　处：《中国环境科学》2017年第2期702-710,共9页China Environmental Science

基　　金：国家"863"项目(2014AA06A509);国家自然科学基金资助目(31370508);中国科学院青年创新促进会(2014273);湖泊与环境国家重点实验室开放研究基金(2012SKL005)

摘　　要：在巢湖开展蓝藻分解的原位围隔实验,实验期间测定围隔中层水体溶解性甲烷浓度和主要理化因子,通过定量PCR的方法测定产甲烷菌mcrA基因拷贝数以及细菌和古菌16S rRNA基因拷贝数随时间的变化情况.结果显示:随着蓝藻有机质分解,水体产甲烷菌数量逐渐增加,溶解性甲烷浓度最高达到2.94mg/L;产甲烷菌mcrA基因拷贝数与甲烷浓度具有显著的正相关关系;同时还发现水体中的亚铁离子(Fe^(2+))含量与产甲烷菌的数量具有显著的正相关关系.上述结果支持蓝藻水华爆发导致水体也成为甲烷生物合成热点区域的观点.本研究获得的结果有助于进一步分析不同产甲烷热点区域(水体和沉积物)对湖泊甲烷总体释放量的贡献情况.In shallow eutrophic lakes,methane production during the decomposition of organic matter derived fromcyanobacterial blooms and associated factors for the whole process in the water column remain poorly understood.In thiswork,in situ mesocosms experiment in Lake Chaohu were carried out to stimulate the Cyanobacteria anaerobicdecomposition.During the process,dissolved methane concentration and the main environmental factors were measuredat the water bottom.Quantitative real-time PCR method was applied to analyze the abundance of mcrA gene as well as thebacterial and archaeal16S rRNA genes in each sample.Results showed that with the biomass decomposition,the amountof methanogens increased gradually in accordance with the accumulation of dissolved methane in the water column(maximum concentration2.94mg/L).A significantly positive correlation was detected between mcrA gene abundance andthe dissolved methane concentration.Furthermore,there was also a significantly positive correlation between ferrous ions(Fe2+)concentration and the methanogen mcrA abundance.Our results support for the notion that water column can be ahot spot for the biosynthesis of methane due to the outbreak of cyanobacterial bloom.This study helps to compare therelative contribution of methane production between water column and surface sediments during the cyanobacterialblooms degradation.

关 键 词：甲烷 产甲烷菌 蓝藻水华 厌氧分解 巢湖 

分 类 号：X524[环境科学与工程—环境工程]