以关联分析发掘烟草抗赤星病基因分子标记  

Identification of Molecular Markers for Tobacco Brown Spot Resistant Genes through Association Analysis

作　　者：朱承广 任民[1] 蒋彩虹[1] 张雨生[1] 孙明铭 刘旦[1] 程立锐[1] 杨爱国[1] 王元英[1] ZHU Chengguang;REN Min;JIANG Caihong;ZHANG Yusheng;SUN Mingming;LIU Dan;CHENG Lirui;YANG Aiguo;WANG Yuanying(Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101

出　　处：《中国烟草科学》2017年第1期68-72,共5页Chinese Tobacco Science

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC01);中国烟草总公司烟草基因组计划重大专项"烟草重要性状基因发掘及功能标记开发"(110201301008);"烟草抗CMV和赤星病连锁分子标记开发及NC82;K326品种抗性改良"(110201301009)

摘　　要：筛选与抗赤星病位点紧密关联的分子标记,为烟草分子标记辅助育种提供依据。利用404对SSR引物对96份烟草种质材料进行基因组扫描,分析其群体结构,并进行烟草赤星病抗性关联分析。结果表明,在两种不同环境条件下共检测到38个与烟草赤星病抗性显著关联的分子标记(P≤0.01),其中标记PT60236、PT50668、PT50176、PT53770、PT60080和PT50727关联强度较大或在不同环境条件下被重复关联到,分别位于6号、8号、9号、19号、3号和23号烟草连锁群上。关联到的分子标记可以用于烟草抗赤星病育种的辅助选择。In order to screen for SSR markers associated with tobacco brown spot resistant genes and to provide useful informationfor breeders,404SSR markers were used to scan polymorphism among a population containing96tobacco germplasms in this study.Population structure was analyzed and association analysis between disease index and SSR markers were performed using TASSEL2.1.38SSR markers were identified as being associated with resistant genes at the level of P≤0.01.The results also indicated thatPT60236,PT50668,PT50176,PT53770,PT60080and PT50727were tightly associated with resistant genes.These SSR markers werelocated in the6th,8th,9th,19th,3th,23th tobacco linkage group respectively.The markers identified in this study are useful inmolecular marker assisted selection in tobacco breeding programs for brown spot resistance.

关 键 词：烟草 赤星病 SSR 关联分析 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]