遗传标记在罗非鱼良种选育研究中的应用与展望  

Application and Prospect of Genetic Markers in Tilapia Breeding

作　　者：颉晓勇[1] 钟金香[2] 赵金良[3] XIE Xiao-yong;ZHONG Jin-xiang;ZHAO Jin-liang(Key Lab. of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Guangzhou 510300, PRC;Fishery Technical Extension Center of Guangdong,Guangzhou 510220, PRC;Shanghai Ocean University, Shanghai 201306, PRC)

机构地区：[1]广东省渔业生态环境重点实验室,中国水产科学研究院南海水产研究所,广东广州510300 [2]广东省水产技术推广总站,广东广州510220 [3]上海海洋大学,上海201306

出　　处：《湖南农业科学》2017年第2期123-126,共4页Hunan Agricultural Sciences

基　　金：国家“十五”攻关项目(2001BA505B0513);中央级公益性科研院所基本科研业务费专项资金(2007TS04)

摘　　要：综述了在近些年中传统遗传标记罗非鱼良种选育相关研究中的应用情况,探讨了多种分子标记联合分析的必要性,展示了罗非鱼选育群体基于SSR/AFLP分别计算的Nei(1978)相似性系数比率稳定分布于0.942 07~0.963 70之间,而群体间SSR/AFLP遗传距离比率全都远大于1,具有良好的区分度。综合多种因素,推荐SSR作为罗非鱼良种选育群体遗传多样性水平的常规监测手段。并指出基于DNA序列测定与生物信息学技术开发的新型分子标记类型应当作为未来罗非鱼良种选育研究的重点方向。In this paper, the application of the traditional genetic marker tilapia breeding in recent years was reviewed. The necessity of the combined analysis of several molecular markers was pointed out. The similarity coefficient ratios of Nei (1978) calculated respectively by SSR/AFLP were between 0.942 07 and 0.963 70 in the tilapia breeding population, meanwhile the genetic distances ratios of Nei (1978) calculated by SSR/AFLP among populations were much larger than 1, which revealed the discrimination ability of SSR. Based on several factors, SSR was recommended as a routine monitoring method for genetic diversity of Tilapia breeding. And it was pointed out that the new type of molecular marker based on DNA sequencing and bioinformatics development should be the focus of the future study of tilapia breeding.

关 键 词：罗非鱼 遗传标记 良种选育 综述 

分 类 号：Q347[生物学—遗传学]