欧美杨111号组织培养再生体系的建立  

Establishment of tissue culture regeneration system of Populus euramericana 'Bellotto'

作　　者：矫丽曼 JIAO Liman(Liaoning Provincial Institute of Populus,Gaizhou 115200,China)

机构地区：[1]辽宁省杨树研究所,辽宁盖州115200

出　　处：《辽宁林业科技》2017年第1期15-18,共4页Liaoning Forestry Science and Technology

摘　　要：以欧美杨111号叶片为外植体,以MS和1/2MS为基本培养基,附加不同浓度的NAA、6-BA和IBA,观察不定芽诱导和生根情况,确定欧美杨111号植株再生体系。结果显示:欧美杨111号叶片最佳分化培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+蔗糖25 g·L^(-1)+琼脂6 g·L^(-1),pH值6.5,不定芽分化率为96.67%;最佳生根培养基为1/2MS+IBA 0.3 mg·L^(-1)+蔗糖20 g·L^(-1)+琼脂6 g·L^(-1),pH值6.0,全部生根,平均根数13.2条。In order to establish plant regeneration system of Populus euramericana‘Bellotto’by tissue culture,leaves of P. euramericana‘Bellotto’were used as explants,MS and 1/2MS were used as basic culture medium,which added with different concentrations of NAA,6-BA and IBA,and the adventitious bud induction and rooting situations were observed. Results showed that: the optimum medium for adventitious bud differentiation was MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+sucrose 25.0 mg·L-1+agar 6.0 mg·L-1,pH 6.5;the optimum medium for rooting was1/2MS+IBA0.3 mg·L-1+sucrose 20.0 mg·L-1+agar 6.0 mg·L-1,pH 6.0.

关 键 词：欧美杨111号 组织培养 再生体系 外植体 

分 类 号：S722.37[农业科学—林木遗传育种]