灵芝孢子油口服乳剂对荷瘤化疗小鼠的增效减毒作用  

The efficacy enhancing and toxicity reducing of ganoderma lucidum spore oil emulsion to mice undergoing chemotherapy

作　　者：刘国健[1] 李菁[2] 陈路林[2] 蒋兆健[2] 刘培庆[3] 陈健文[3] 郭丽冰[1] LIU Guo-jian;LI Jing;CHEN Lu-lin;JIANG Zhao-jian;LIU Pei-qing;CHEN Jian-wen;GUO Li-bing(Guangdong Pharmaceutical University, Guangzhou 510006, China;National Engineer Research Center for the Modernization of Extraction and Separation of TCM/Guangzhou Han Fang Pharmaceutical Company Limited, Guangzhou 510240,;Laboratory of Pharmacology and Toxicology, Schoool of Pharmacy, Sun Yat-sen University, Guangzhou 510006, China)

机构地区：[1]广东药科大学,广州510006 [2]广州白云山汉方现代药业有限公司/中药提取分离过程现代化国家工程研究中心,广州510240 [3]中山大学药学院药理与毒理学实验室,广州510006

出　　处：《肿瘤代谢与营养电子杂志》2017年第1期60-66,共7页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：广州市药用脂质重点实验室([2013]163-8)

摘　　要：目的研究灵芝孢子油乳剂口服给药的抑瘤及增效减毒作用。方法实验分为对照组、CP单独给药组、CP+灵芝孢子油口服乳低剂量组、CP+灵芝孢子油口服乳高剂量组、灵芝孢子油口服乳低剂量组、灵芝孢子油口服乳高剂量组、康莱特软胶囊组、CP+康莱特软胶囊组,共8组,每组10只,将H22细胞株接种小鼠腋下靠背部成荷瘤状态,同时口服给药治疗21天,观察灵芝孢子油口服乳结合化疗药对肿瘤生长抑制作用。结果灵芝孢子油乳剂口服给药联合常规化疗药物CP使用,有增强化疗药物抑制肿瘤生长的作用趋势;可改善化疗药对WBC、RBC、HGB的降低作用。其中CP+灵芝孢子油口服乳高剂量组明显升高(P<0.05);可减轻化疗药对肝功能的损害;有改善化疗药物对脾系数、胸腺系数、BMNC、网织红细胞、脾结节降低或减少的作用。结论灵芝孢子油口服乳剂联合化疗药物CP使用,具有一定的肿瘤抑制和增效减毒作用,其作用机制可能与其具有增强免疫力、促进骨髓造血功能有关。Objective To investigate the anti-tumor effect of oral administration of ganoderma lucidum spores oil emulsion and its synergism and attenuation.Methods In this study,eighty mice were divided into8groups:control group,CP,CP+ganoderma lucidum spore oil oral emulsion,low dose and high dose;ganoderma lucidum spore oil oral emulsion,low dose and high dose;Kanglaite soft capsule;CP+Kanglaite soft capsule,with10rats in each group.To creating tumor bearing model,H22cell line was inoculated into the back of the axilla of the mice.Giving administration in the treatment of ganoderma lucidum spores oil oral emulsion combined with chemotherapy drugs for21days,and observed whether tumor growth was inhibited.Results For oral administration of ganoderma lucidum spores oil emulsion combined with conventional chemotherapy drugs CP,there is a trend toenhance the effect of chemotherapy drugs to inhibit tumor growth.It can also improve the side effect of chemotherapy drugs onWBC,RBC,HGB.WBC(P<0.05)which was significantly increased in the high dose group of CP+ganoderma lucidum spore oilemulsion.Ganoderma lucidum spores oil emulsion can reduce the damage of chemotherapy drugs on liver function.To improve the effect of chemotherapy drugs on the spleen index,thymus coefficient,the number of bone marrow cells,the number of cells in the reticulated red blood cells,and the reduction of the splenic nodules.Conclusions Ganoderma lucidum spores oil oral emulsion combined with chemotherapy drug CP,with certain tumor suppressor and attenuated synergistic effect and its mechanism may be related to enhance immunity,promote hematopoietic function of bone marrow.

关 键 词：灵芝孢子油口服乳剂 抑瘤作用 增效减毒作用 

分 类 号：R285.5[医药卫生—中药学]