高溶氧对西伯利亚鲟幼鱼非特异性免疫指标的影响  

Effects of Hyperoxia on Non-specific Immunology in Acipenser Baeri

作　　者：王晓雯[1] 朱华[1] 马国庆[1] WANG Xiao-wen;ZHU Hua;MA Guo-qing(Beijing Key Laboratory of Fishery Biotechnology,Beijing Fisheries Research Institute,Beijing 100068,China)

机构地区：[1]北京市水产科学研究所,渔业生物技术北京市重点实验室,北京100068

出　　处：《四川农业大学学报》2017年第1期93-98,共6页Journal of Sichuan Agricultural University

基　　金：国家科技支撑计划项目(2015BAD25B01);现代农业产业技术体系北京市鲟鱼、鲑鳟鱼创新团队项目(SCGWZJ20161104);中央引导地方科技发展专项(名优水产良种选育与养殖技术推广Z161100004516003)

摘　　要：【目的】为揭示高溶解氧对西伯利亚鲟幼鱼非特异性免疫指标的影响。【方法】分别设置了5个溶氧水平组(为6、8、10、12、14 mg/L),在养殖试验第100 d分析了西伯利亚鲟外周血白细胞的吞噬活力、脾脏细胞吞噬活性、血清与黏液中溶菌酶活性、碱性磷酸酶活性和酸性磷酸酶活性以及血清杀菌活力。【结果】西伯利亚鲟的血清杀菌活力、脾脏巨噬细胞的呼吸爆发活力和外周血白细胞吞噬活力分别在10、10、12 mg/L下达到峰值,而肝脏酸性磷酸酶活力无明显差异,8、10、12、14 mg/L下鲟鱼碱性磷酸酶活力略微低于6 mg/L组。【结论】在6~12 mg/L范围内适当提高溶解氧含量可刺激西伯利亚鲟鱼血清和脾脏的杀菌活力,但肝脏中碱性磷酸酶参与的免疫防御功能受到略微影响。【Objective】Evaluate the effects of hyperoxia on non-specific immunity of juvenile sturgeon.【Methods】Acipenser baeri juveniles with body weight of(20.83±0.21)g were raised in different fivedissolved oxygen level(6,8,10,12,14mg·L-1).The serum immune indicators,activities of lysozyme,ACP,AKP and antibacterial in mucus and serum were analyzed at100d after the hyperoxia stress.【Results】The results showed that the antibacterial activity of serum,leukocytes phagocytic activity,respiratory burst of macrophage in spleen respectively reached maximum at group of10mg·L-1,10mg·L-1,12mg·L-1.What’s more,the activity of ACP in liver of all groups did not have remarkabledifferences,and activities of AKP in8,10,12,14mg·L-1group were slightly lower than in6mg·L-1group.【Conclusion】In conc-lusion,some level of high dissolved oxygen within6~12mg·L-1couldpromote the antibacterial activity of serum and spleen to a certain degree.However,the immune defensefunction of alkaline phosphatase in liver was influenced slightly.

关 键 词：西伯利亚鲟 高溶氧 吞噬活力 碱性磷酸酶 酸性磷酸酶 杀菌活力 

分 类 号：S943[农业科学—水产养殖]