山东地区棕榈蓟马对甜瓜黄斑病毒的传播  

Melon yellow spot virus(MYSV)Transmitted by Thrip palmi in Shandong

作　　者：代惠洁[1,2] 刘锦[2] 刘永杰[2] 乔宁[2] 马井玉[3] 张安盛[4] Dai Hui-jie;Liu Jin;Liu Yong-jie;Qiao Ning;Ma Jing-yu;Zhang An-sheng(Weifang University of Science and Technology,Shouguang 262700,Shandong,China;College of Plant Protection,Shandong Agricultural University,Tai’an 271018,Shandong,China;Jining Academy of Agricultural Sciences,Jining 272100,Shandong,China;Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China)

机构地区：[1]潍坊科技学院,山东寿光262700 [2]山东农业大学植物保护学院,山东泰安271018 [3]济宁市农业科学研究院,山东济宁272100 [4]山东省农业科学院植物保护研究所,山东济南250100

出　　处：《中国蔬菜》2017年第4期61-64,共4页China Vegetables

基　　金：公益性行业(农业)科研专项(201303028);山东省农业重大应用技术创新项目

摘　　要：在山东济南董家镇温室内发现疑似感染甜瓜黄斑病毒(Melon yellow spot virus,MYSV)的黄瓜植株,通过RT-PCR检测,获得大约500bp的特异性片段。经测序确认,该病毒为MYSV;对发病植株温室内采集的棕榈蓟马进行RT-PCR检测,发现33.3%的棕榈蓟马体内携带MYSV。室内试验中,MYSV感病株率随着带毒棕榈蓟马数量和取食时间增加而明显升高,表明棕榈蓟马能有效传播MYSV,其种群数量和取食时间对MYSV的传播具有显著的影响,生产中可通过防控棕榈蓟马预防MYSV的传播。Cucumber samples infected possibly by Melon yellow spot virus(MYSV)were found in Jinan,Shandong.A Reverse Transcript-PCR assay was performed with total RNA isolated from diseased samples byspecific primers for MYSV,and amplified DNA fragments(500bp)were obtained.After sequencing,we confirmed that the diseased samples were infected by MYSV.A Reverse Transcript-PCR assay was performed with Thrip palmi collecting in the greenhouse,and the results showed that33.3%of Thrip palmi carried MYSV,meaning that Thrip palmi was vector of MYSV.MYSV incidence rate increased with viruliferous Thrip palmipo pulation increasing and feeding time extending.The results indicated that MYSV could be effectively transmitted by Thrip palmi,and the prevention and control of Thrip palmi was an effective measure for controlling MYSV in the fields.

关 键 词：甜瓜黄斑病毒 棕榈蓟马 传毒 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]