HBV相关肝炎、肝硬化及肝癌患者DcR3的表达及其与Fas的关系  

Relationship of DcR3 expression with Fas expression in patients with chronic hepatitis B,liver cirrhosis and liver cancer

作　　者：梁冬雨[1] 侯彦强[1] 娄晓丽[1] LIANG Dongyu;HOU Yanqiang;LOU Xiaoli(Department of Central Laboratory,Songjiang Campus,Shanghai General Hospital,Shanghai Jiaotong University,Shanghai 201600,China)

机构地区：[1]上海交通大学附属第一人民医院松江分院中心实验室,上海201600

出　　处：《检验医学》2017年第3期178-181,共4页Laboratory Medicine

基　　金：国家自然科学基金青年科学基金项目(81401937)

摘　　要：目的研究乙型肝炎病毒(HBV)相关肝炎、肝硬化及肝癌患者诱骗受体3(DcR3)的表达情况及其与死亡受体Fas的关系。方法常规检查34例慢性乙型肝炎患者、31例HBV相关肝硬化患者、28例HBV相关肝癌患者和28名健康体检者(正常对照组)的丙氨酸氨基转移酶(ALT)水平及HBV DNA载量,其中10例行肝组织病理检查。采用酶联免疫吸附试验(ELISA)检测血清中DcR3的水平,并与ALT、HBV DNA载量作相关性分析。采用荧光定量聚合酶链反应(PCR)检测肝组织中DcR3和Fas mRNA的水平,并作相关性分析。结果慢性乙型肝炎组、HBV相关肝硬化组、HBV相关肝癌组与正常对照组比较,血清DcR3水平明显升高(P<0.05),HBV相关肝癌组较其他组升高更明显(P<0.05),其余各组间差异无统计学意义(P>0.05)。慢性乙型肝炎患者血清DcR3水平与ALT、HBV DNA呈正相关(r值分别为0.448 1、0.500 7,P<0.05)。HBV相关肝癌患者癌组织中Fas mRNA表达降低(P<0.05),且与DcR3 mRNA表达呈负相关(r=-0.794 1,P<0.05)。结论 DcR3可能通过Fas参与HBV相关肝癌的发生和发展。Objective To investigate the expression of serum decoy receptor 3 (DcR3) in patients with chronic hepatitis B,liver cirrhosis and liver cancer and its relationship with Fas expression. Methods Alanine aminotransferase (ALT)and hepatitis B virus(HBV) DNA load were determined in 34 patients with chronic hepatitis B,31 patients with liver cirrhosis,28 patients with liver cancer and 28 healthy subjects (healthy control group). A total of 10 of the patients underwent liver tissue pathological examination. Serum DcR3 levels were determined by enzyme-linked immunosorbent assay (ELISA),and the correlations of serum DcR3 levels with ALT and HBV DNA load were analyzed. The levels of serum DcR3 and Fas mRNA in liver tissue were determined by fluorescence quantitation polymerase chain reaction (PCR),and the correlation analysis was performed.Results Compared with healthy control group,serum DcR3 levels in 3 patient groups all increased (P<0.05).Serum DcR3 levels became higher in liver cancer group than the other 2 patient groups(P<0.05). There was no statistical significance between chronic hepatitis B and liver cirrhosis groups(P>0.05). Serum DcR3 levels were correlated with ALT and HBV DNA load(r=0.448 1 and 0.500 7,P<0.05). The expression of Fas mRNA decreased in liver tissue in liver cancer group(P<0.05). There was a negative correlation between the expressions of serum DcR3 and Fas mRNA(r=-0.794 1,P<0.05). Conclusions DcR3 affects the development of HBVrelated liver cancer through Fas .

关 键 词：诱骗受体3 FAS受体 慢性乙型肝炎 肝硬化 肝癌 

分 类 号：R446.62[医药卫生—诊断学]