陕西省咸阳地区人乳头瘤病毒的基因分型及亚型分布特征  

Genotyping and subtype distribution of human papillomavirus in Shaanxi Xianyang

作　　者：李军[1] 王一羽[1] 南星[1] 王国庆[2] 柳瑛[1] 原荣[1] 尹悦[1] 田小飞[2] LI Jun;WANG Yiyu;NAN Xing;WANG Guoqing;LIU Ying;YUAN Rong;YIN Yue;TIAN Xiaofei(Basic Research Center,Shaanxi Provincial Tumor Hospital,Xi'an Jiaotong University Health Science Center,Xi'an 710061,Shaanxi,China;Gynecological Cancer Research Center,Shaanxi Provincial Tumor Hospital,Xi'an Jiaotong University Health Science Center,Xi'an 710061,Shaanxi,China)

机构地区：[1]西安交通大学医学院附属陕西省肿瘤医院基础研究中心,陕西西安710061 [2]西安交通大学医学院附属陕西省肿瘤医院妇科肿瘤中心,陕西西安710061

出　　处：《检验医学》2017年第3期194-198,共5页Laboratory Medicine

基　　金：陕西省科技计划发展项目(2011K15-06-09)

摘　　要：目的对陕西省咸阳地区妇女人乳头瘤病毒(HPV)感染及亚型分布特征进行研究。方法采用聚合酶链反应(PCR)-反向点杂交技术对7 708例宫颈脱落细胞样本进行HPV的基因分型检测。结果在被研究的7 708例妇女中感染HPV者2 477例,总感染率为32.14%(2 477/7 708),高危型HPV感染率(包括高危多重感染)为23.96%(1 847/7 708),在被检测的18种高危亚型中,常见的类型为16、52、58、51、56和66型。HPV单一基因型的感染率为27.58%(2 126/7 708),单一高危型的感染率为19.94%(1 537/7 708),单一高危型感染常见的亚型为16、52、58、51、33和39型。多重感染率为4.55%(351/7 708),多重高危型的感染率为4.53%(349/7 708)。在不同年龄组妇女中,31~40岁组感染率最高(10.20%)。在31~40岁组妇女HPV高危型感染的亚型主要为16、52、58、51、56和33型,其他各年龄组感染的亚型分布各不相同。结论陕西省咸阳地区妇女的宫颈HPV感染率较高,常见高危型为16、52、58、51、56和66型。各年龄段妇女感染HPV的亚型分布特征各不相同,30~50岁年龄段妇女应作为HPV监测的重点人群。Objective To investigate the infection status and subtype distribution of human papillomavirus(HPV) in Shaanxi Xianyang. Methods A total of 7 708 cervical cell specimens were analyzed by polymerase chain reaction (PCR)- reverse spot hybridization technology for HPV genotyping. Results A total of 2 477 patients with HPV infection were determined in 7 708 women. The total infection rate was 32.14%(2 477/7 708),and the infection rate of high-risk subtypes was 23.96%(1 847/7 708),including multiple high-risk subtypes. The most common high-risk subtypes were 16,52,58,51,56 and 66 in the 18 high-risk subtypes. The infection rate of single subtype in patients with HPV infection was 27.58% (2 126/7 708). The infection rate of single high-risk subtype was 19.94%(1 537/7 708). The common single high-risk subtypes were 16,52,58,51,33 and 39.The infection rate of multiple subtype was 4.55%(351/7 708). The infection rate of multiple high-risk subtype was4.53% (349/7 708). The infection rate of 31-40 years old group was the highest (10.20%). The common highrisk subtypes were 16,52,58,51,56 and 33 in 31-40 years old group. There was different subtype distribution in other age groups. Conclusions The infection rate of HPV in Shaanxi Xianyang is high,and the common high-risk subtypes are 16,52,58,51,56 and 66. HPV subtype distribution of different ages has a certain difference. The women of 30-50 years old should be monitored for HPV.

关 键 词：人乳头瘤病毒 基因分型 聚合酶链反应-反向点杂交技术 陕西省 咸阳地区 

分 类 号：R446.5[医药卫生—诊断学]