肠易安汤对溃疡性结肠炎大鼠溃疡组织中NF-κB p65及IL-10表达的影响  

Effect of Changyian on the expression of nuclear factor kappa B and interleukin-10 in ulcerative colitis rats

作　　者：马春花[1] 罗京艺[1] 朱东华[1] 荆淑娟 尚毅[1] Ma Chunhua;Luo Jingyi;Zhu Donghua;Jing Shujuan;Shang Yi(Department of Anorectal Surgery,Liaocheng Second People′s Hospital, Shandong 252600, China)

机构地区：[1]山东省聊城市第二人民医院肛肠科,252600

出　　处：《中华结直肠疾病电子杂志》2017年第2期117-120,共4页Chinese Journal of Colorectal Diseases(Electronic Edition)

摘　　要：目的观察中药方"肠易安汤"对实验性大鼠溃疡性结肠炎结肠粘膜组织NF-κB p65、白细胞介素-10(IL-10)表达的影响。方法采用2,4,6-三硝基苯磺酸(TNBS)加免疫诱导法复制UC大鼠模型,39只大鼠,随机分组为模型组,肠易安汤组,柳氮磺胺吡啶(SASP)组。治疗21 d后观察大鼠结肠组织黏膜的病理改变(CMDI)、NF-κB p65、IL-10表达水平的变化。结果治疗21 d后,肠易安汤组大鼠CMDI评分和NF-κB p65表达明显低于模型组(t=58.28,P=0.003;t=6.01,P=0.016)和SASP组(t=-22.10,P=0.026;t=-2.80,P=0.038),肠易安汤组IL-10表达明显高于模型组和SASP组(t=-403.56,P=0.022;t=35.76,P=0.036)。结论肠易安汤对溃疡性结肠炎大鼠结肠组织黏膜有良好的修复作用,其作用机理可能与降低NF-κB p65表达和提高IL-I0水平有关。Objective To observe the effect of Changyian on the expression of NF-κB p65andinterleukin-10in ulcerative colitis rats.Methods Ulcerative colitis rat model was copied by2,4,6-TNBS.Thirty-nine rats were randomly divided into3groups:model group,Changyian group and salazosulfapyridine(SASP)group.After21days′treatment,the changes of colonic mucosal damage index(CMDI),levels of NF-κBp65and IL-10were observed.Results After21days′treatment,the CMDI and the expression of NF-κBp65of Changyian group was significantly lower than the model group(t=58.28,P=0.003;t=6.01,P=0.016)and SASP group(t=-22.10,P=0.026;t=-2.80,P=0.038).The expression of interleukin-10was obviouslyhigher than that of model group and SASP group(t=-403.56,P=0.022;t=35.76,P=0.036).ConclusionChangyian has good repair effect on ulcerative colitis rats colon mucosa.Its mechanism may be related toreduce the expression of NF-κB p65and improve the level of IL-I0.

关 键 词：结肠炎 溃疡性 白介素-10 核因子ΚB P65 肠易安汤 

分 类 号：R285.5[医药卫生—中药学]