量子点复合微球的活体荧光和化学发光双模式成像  

Quantum Dots Composite Microspheres for In Vivo Fluorescence and Chemiluminescence Dual-Mode Imaging

作　　者：叶一[1] 王国武[1] 王莉萍[1] 和田智之[2] 王瑾晔[1] YE Yi;WANG Guo-wu;WANG Li-ping;WADA Satoshi;WANG Jin-ye(School of Biomedical Engineering,Shanghai Jiao Tong University, Shanghai 200240, China;Center for Advanced Photonics, RIKEN, Saitama Wako 351-0198, Japan)

机构地区：[1]上海交通大学生物医学工程学院,上海200240 [2]日本理化学研究所先进光子学中心

出　　处：《中国医疗设备》2017年第4期26-29,共4页China Medical Devices

基　　金：国家自然科学基金(30470477;30870635);科技部国家国际科技合作计划项目(2015DFG32730);上海市科学技术委员会国际合作重点项目(15540723900)

摘　　要：为了探索荧光/化学发光双模式成像方法用于可降解生物材料研究的可行性,将载有量子点的可降解高分子微球(量子点复合微球)植入小鼠背部皮下,利用美国Caliper定量荧光和生物发光成像系统IVIS Lumina II观察不同时间点植入部位的荧光强度和L-012介导的化学发光强度的变化。结果显示:30 mg量子点复合微球植入28 d时,仍能检测到荧光信号。L-012介导的化学发光强度随时间逐渐减弱,第28 d时,量子点复合微球处仍存在微弱的化学发光。To develop in vivo fluorescence and chemiluminescence dual mode imaging method for materials, quantum dots (QDs) loaded with biodegradable polymer microspheres (BPM) were subcutaneously injected into the back of mice. L-012 was employed to noninvasively monitor the product of reactive oxygen species. The fluorescence intensity and chemiluminescence intensity were observed by Quantitative Fluorescent and Bioluminescent Imaging System IVIS Lumina II from Caliper at different time points. The results showed that the fluorescence of 30 mg BPM/QD could be detected up to 28 d.Meanwhile, chemiluminescence intensity of the implanted microspheres induced by L-012 decreased with time. On day 28, there was still weak chemiluminescence where the microspheres were implanted.

关 键 词：量子点 复合微球 活体荧光成像 活性氧 化学发光 

分 类 号：R318.08[医药卫生—生物医学工程]