绿潮藻浒苔微生物高效降解及生物乙醇制备  

Screening and characterization of a newly isolated strain for microbiological degradation of Ulva prolifera and bioethanol production

作　　者：王淑贤[1,2] 韦章良 贾睿[1,2] 张建恒[4] 霍元子[1,2] 于克锋[1,2] 何培民[1,2] WANG Shu-xian;WEI Zhang-liang;JIA Rui;ZHANG Jian-heng;HUO Yuan-zi;YU Ke-feng;HE Pei-min(College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Water Environment and Ecology Engineering Center of Shanghai Institute of Higher Education, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海海洋大学水域环境生态上海高校工程研究中心,上海201306 [3]中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室,广东广州510301 [4]上海海洋大学海洋科学学院,上海201306

出　　处：《海洋科学》2017年第1期76-82,共7页Marine Sciences

基　　金：国家自然科学基金(41576163);国家重点研发计划项目(2016YFC1402105);国家海洋局公益性科研专项(201205010;201105023);科技支撑计划项目(2012BAC07B023)~~

摘　　要：从绿潮藻暴发海域的底泥和腐烂浒苔(Ulva prolifera)中,筛选出一株可高效降解浒苔纤维素的微生物菌株(F菌株),通过刚果红染色实验以及真菌的ITS分析,鉴定为曲霉属真菌。以浒苔为诱导培养基制备F菌株的粗酶液,测得其滤纸纤维素酶活为34.79 U/m L。在发酵实验中,当酶解条件为6%的粗酶液添加量、反应温度为38℃、反应时间为60 h、pH为6.8时,浒苔纤维素降解效果最好,酶解液发酵乙醇产量最高达到28.98 g/L。Ulva proliferahas been the dominant species in the huge annual biomass accumulation during green algae bloom in Yellow Sea since2007.This species can be used for energy utilization by fermentation under certain conditions.In this study,we identified ahighlyefficient cellulose-decomposing microorganismbyscreeningthe rotten algae and employedit as a raw material to manufacture bioethanol.We studied its cellulose activity and the ability to decomposeU.prolifera atdifferentreaction temperatures,times.pH,and dosages of crude enzyme.We identified the strain with highest efficiency in cellulose-decomposition using Congo red staining method and ITSand termed it as theF strain(34.79U/mL).We also foundthatthe optimum conditionsfor the fermentation of U.prolifera were as follows:crude enzyme,6%;treating temperature,38°C;treating time,60h;and pH,6.8.Under these optimum conditionsbioethanol yield of up to28.98g/Lwas achieved.

关 键 词：浒苔(Ulvaprolifera) 纤维素降解菌 发酵 生物乙醇 

分 类 号：P745[天文地球—海洋生物学]