莠去津高效降解菌的分离与鉴定  

Isolation and Identification of Atrazine High Efficient Degradation Bacteria

作　　者：吕惠萍[1] LYU Huiping(Department of Chemical Engineering, Heze College, Heze 274015, China)

机构地区：[1]菏泽学院化学化工系,山东菏泽274015

出　　处：《化工技术与开发》2017年第2期22-24,共3页Technology & Development of Chemical Industry

摘　　要：笔者从长期使用莠去津的农田和果园的土壤中分离到3株莠去津降解菌,编号为AT-2、AT-3、AT-4。通过生理生化指标和形态分析,对AT-2以及本实验室现有的3株降解菌HB-1、HB-7、HW-4进行初步鉴定,分别为土壤杆菌属、布鲁氏菌属、葡萄球菌属、假单胞菌属。测定这4株菌的降解率,反应条件为:底物莠去津浓度为100mg·L^(-1),反应体系5mL,5%的接菌量,恒温30℃,180r·min^(-1),培养24h,其降解效率分别为34.2%、37.3%、43%和40%,但在3d后,4株菌的降解率可达到90%左右。3atrazine degradation bacterium strains were isolated from the farmland and orchard soil of long-term use of atrazine,and marked as AT-2,AT-3and AT-4.AT-2and3strains of this laboratory existing degradation bacteria HB-1,HB-7and HW-4,were preliminary identified by physiological and biochemical indexes and morphological analysis,and they were soil bacillus genus brucella,staphylococcus,pseudomonas,respectively.Bacteria degradation rate of the four strains was determined.The reaction conditions were:the substrate concentration of atrazine was100mg/L,reaction system5mL,5%of inoculation quantity,constant temperature30℃,180r/min,cultivate24h,the degradation efficiency were34.2%,37.3%,43%and34.2%,respectively.After3d,degradation rate of4isolates could reach about90%.

关 键 词：莠去津 降解菌 降解率 

分 类 号：TQ457.2[化学工程—农药化工]