山莨菪碱对心搏骤停大鼠心肌和脑组织代谢影响的研究  

The effects of anisodamine on myocardial and brain tissues functions of cardiac arrest rats

作　　者：贾立静[1] 孟凡山[2] 李坤[1] 李天然[3] 陈威[1] 计达[1] 沈洪[1] JIA Li-jing;MENG Fan-shan;LI Kun;LI Tian-ran;CHEN Wei;JI Da;SHEN Hong(Department of Emergency, General Hospital of PLA, Beijing 100853, China;No. 88 Hospital of PLA, Taian 271000, China;Department of Nuclear Medicine, PLA General Hospital, Beijing 100853, China)

机构地区：[1]解放军总医院急诊科,北京100853 [2]解放军第88医院,山东泰安271000 [3]解放军总医院核医学科实验室,北京100853

出　　处：《中国药物应用与监测》2017年第2期84-87,共4页Chinese Journal of Drug Application and Monitoring

基　　金：公益性行业科研专项立项基金课题(201502019)

摘　　要：目的:观察山莨菪碱对心搏骤停大鼠心肌和脑组织代谢的作用。方法:采用交流电生理刺激仪经食道调搏诱发心脏骤停模型。65只SD大鼠随机分为5组:生理组(n=5)、生理盐水组(对照组,n=15)、肾上腺素组(Epi组,n=15),山莨菪碱组(Ani组,n=15),肾上腺素联合山莨菪碱组(联合组,n=15)。采用盲法给药,调搏前静脉注射2-[^(18)F]氟-2-脱氧-D-葡萄糖(FDG)0.6 m L。复苏后30 min内留取部分实验大鼠心脏及脑组织进行放射性计数,留取静脉血送检血浆心房利尿钠肽(ANP),并计算自主循环恢复率和复苏成功率。结果:肾上腺素联合山莨菪碱组复苏成功率为70%,高于其它各组,差异有统计学意义(P<0.01)。肾上腺素复苏成功的心脏和脑组织放射性计数均明显低于肾上腺素联合山莨菪碱组,两组心脏和脑组织放射性计数数值分别为(25.71±2.83 vs 38.3±7.2,P<0.05)、(16.06±2.93 vs 31.02±2.72,P<0.05),联合组及山莨菪碱组复苏成功大鼠血浆ANP水平明显低于肾上腺素组,差异具有统计学意义(P<0.05),但均高于生理组,差异具有统计学意义(P<0.05)。结论:复苏早期联合应用山莨菪碱干预可能提高复苏成功率,改善心肌及脑组织的代谢,对心肺复苏大鼠有保护作用。Objective:Observe the effect of anisodamine on functions of myocardial and brain tissues of rats suffering from cardiac arrest.Methods:The cardiac arrest model of rats were induced by alternating current(AC)via pacing electrode placed in esophagus.Five minutes after onset of cardiac arrest,cardiopulmonary resuscitation(CPR)was initiated.A total of65SD rats were randomly divided into five groups as follows:physical group(n=5),physiological saline group(n=15),epinephrine(Epi)group(n=15),anisodamine(Ani)group(n=15),anisodamine and epinephrine combination group(n=15).We injected0.6mL FDG before the stimulation.Medicine was given in blind way.The hearts and brain of the rats were removed for radiocounting,and the plasma A-type natriuretic peptide(ANP),and the rate of restored of self circulation(ROSC)and success resuscitation were also detected.Results:The success rate of resuscitation in Ani and Epi combination group was70%,that was higher than other groups,the difference was significant statistically(P<0.01).The myocardium and the cerebrum radiocounting of the Epi recovery group were lower than those of the Ani and Epi combination recovery group,which of the myocardium were(25.71±2.83vs38.3±7.2,P<0.05)and the cerebrum were(16.06±2.93vs31.02±2.72,P<0.05),respectively.The plasma ANP level of the recovery in the Ani and Epi combination group and the Ani group were lower than that of the Epi group(P<0.05),but all of these groups were higher than that of the physical group,the difference was significant statistically(P<0.05).Conclusion:The early recovery of anisodamine combined with epinephrine may improve the success rate of resuscitation,improve the metabolism of myocardium and brain tissue,and has protective effects on cardiopulmonary resuscitation in rats.

关 键 词：心脏停搏 心肺复苏 山莨菪碱 肾上腺素 心房利尿钠肽 

分 类 号：R541.78[医药卫生—心血管疾病]