知母总皂苷对血管性抑郁小鼠抑郁行为和脑组织病理学的影响  

Effects of saponins from Anemarrhena asphodeloides Bge. on the depressive behaviors of vascular depression mice and brain tissue pathology

作　　者：龚林 邱彦 刘静 鲁毅 王亚威 段靖 马静 周永 GONG Lin;QIU Yan;LIU Jing;LU Yi;WANG Ya-wei;DUAN Jing;MA Jing;ZHOU Yong(Outpatient Department,the No. 454 Hospital of PLA, Nanjing 210002, China;Department of Pharmacy, the No. 454 Hospital of PLA, Nanjing 210002,China;Instrument Section, the No. 454 Hospital of PLA, Nanjing 210002, China)

机构地区：[1]解放军454医院门诊部,江苏南京210002 [2]解放军454医院药剂科,江苏南京210002 [3]解放军454医院器械科,江苏南京210002

出　　处：《中国药物应用与监测》2017年第2期88-92,共5页Chinese Journal of Drug Application and Monitoring

基　　金：南京军区基学科研基金课题(15zD021)

摘　　要：目的:探讨知母总皂苷对血管性抑郁小鼠抑郁行为、脑组织炎症因子及病理学的影响。方法:采用反复缺血再灌注方法制备血管性抑郁小鼠模型,灌胃给予知母总皂苷,实验2周后,观察血管性抑郁小鼠行为学变化,检测小鼠脑组织中炎症因子TNF-α、IL-1β、IL-6的含量以及脑组织病理学改变。结果:与模型组相比,知母总皂苷中剂量组和高剂量组小鼠水平运动得分、垂直运动得分均明显增加(P<0.05),明显缩短小鼠悬尾、游泳不动时间(P<0.05);与模型组比较,知母总皂苷三个剂量组均显著抑制小鼠脑组织中TNF-α、IL-1β、IL-6含量的升高(P<0.05)。脑组织病理切片显示,与模型组相比,知母总皂苷各剂量组可显著减轻大脑皮质锥体细胞和脑实质神经细胞出现的核固缩、核溶解、核体不规则等情况。透射电镜结果显示,与模型组比较,知母总皂苷各剂量组能改善脑组织神经元细胞变小、突触泡扩张、血管周围有空白区等现象。结论:知母总皂苷对血管性抑郁小鼠具有显著的治疗作用。Objective:To investigate the effects of saponins from Anemarrhena asphodeloides Bge.(SAaB)on the depressive behaviors of vascular depression mice,and the influence of brain tissue pathology and inflammatory factors.Methods:The vascular depression model of mice were established by repeated ischemia and reperfusion.The mice received gastric perfusion of SAaB as designed once per day.After2weeks,the depressive behavior changes of vascular depression mice were observed.Contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in brain tissue were measured,meanwhile pathological changes in the brain tissue of micewere analyzed.Results:Compared with the model group,SAaB in middle and high dose group could increase horizontal movement and vertical movement score in open field test(P<0.05).In the tail suspension test and the forced swimming test of mice,the immobility time of SAaB middle and high dose group was shorter than that of the model group significantly(P<0.05).Compared with the model group,SAaB in low,middle and high dose groups could significantly inhibit the increase of TNF-α,IL-1βand IL-6in the mice brain tissue notably(P<0.05).The pathological examination showed that SAaB in each dosage group significantly reduced the fuzzy neurons in the brain structure,mitochondria swelling,nissl body,pycnosis,irregular nuclear body in the cerebral cortex pyramidal cells and brain nerve cell nucleus.Compared with the model group,SAaB in each dosage group improved the brain neurons atrophy,synaptic vesicle expansion,nucleus chromatin edge setting,deformity and the blank area around blood vessels.Conclusion:SAaB has significant therapeutic effect on vascular depression mice.

关 键 词：知母总皂苷 血管性抑郁 行为学 炎症因子 病理学 

分 类 号：R285.5[医药卫生—中药学]