4-氯-7-硝基苯并噁二唑的合成及对次氯酸根的荧光检测  

Synthesis and hypochlorite detection of 4-chlorine-7-nitrobenzeneoxadiazole fluorescent probe

作　　者：刘亮亮[1] 郭雨芳 严奉梅 张春香[1] 申有名[1] Liu Liangliang;Guo Yufang;Yan Fengming;Zhang Chunxiang;Shen Youming(College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China)

机构地区：[1]湖南文理学院化学与材料工程学院,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2017年第2期24-27,共4页Journal of Hunan University of Arts and Science(Science and Technology)

基　　金：湖南省教育厅一般项目(15C0934);湖南省大学生研究性学习和创新实验计划项目;湖南文理学院大学生研创新项目;湖南省十二五重点建设学科-应用化学资助

摘　　要：以2,6-二氯苯胺为原料合成了4-氯-7-硝基苯并噁二唑的荧光探针,其结构经过NMR进行了表征。利用荧光光谱探讨了该探针对次氯酸根的荧光响应性能。实验结果表明,该探针对次氯酸根具有良好的选择性和灵敏性。在V_(CH_3CH_2OH):V_(PBS)=1:1(50 m M PBS buffer,pH7.4)的缓冲溶液中,探针随着次氯酸根浓度的增加而增强,探针对次氯酸浓度在0~16μM范围内具有较好的线性关系,其对次氯酸根的检测下限为0.23μmol/L。4-Chlorine-7-nitro-benzeneoxadiazole fluorescent probe is synthesized from2,6-Dichloroaniline.Itsstructure is characterized by NMR.The spectroscopic properties of the probe with hypochlorite are investigated.Theexperiment shows that the probe is highly selective,sensitive and fast recognition to hypochlorite.Upon addition ofincreasing concentration of hypochlorite,a gradually increases fluorescence intensity of compound1isobserved in EtOH-PBS solution(V/V,50mM PBS buffer solution at pH7.4).The fluorescence intensity isproportional to the concentration of glucose over a range of0～16μM,with a limit of detection of0.23μmol/L.

关 键 词：荧光探针 次氯酸根 苯并噁二唑 

分 类 号：O657.3[理学—分析化学]