青枯雷尔氏菌拮抗放线菌的筛选及其抗菌活性物质的分离  

The Selection of Antagonistic Actinomycetes against Ralstonia solanacearum and Purification of Antibacterial Substance

作　　者：罗文建[1] 刘雨虹[1] 施河丽[2] 谭军[2] 王瑞[2] 赵秀云[1] 向必坤[2] LUO Wenjian;LIU Yuhong;SHI Heli;TAN Jun;WANG Rui;ZHAO Xiuyun;XIANG Bikun(College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;Enshi Tobacco Company of Hubei Province, Enshi, Hubei 445000, China)

机构地区：[1]华中农业大学农业生命科学技术学院,武汉430070 [2]湖北省烟草公司恩施州公司,湖北恩施445000

出　　处：《中国烟草科学》2017年第2期69-74,共6页Chinese Tobacco Science

基　　金：中国烟草总公司科技重点项目"基于宏基因组学的植烟土壤健康评价研究与应用"(110201402016)

摘　　要：烟草青枯病是由青枯雷尔氏菌引起的土传性病害。从健康土壤中分离到对青枯雷尔氏菌具有明显拮抗作用的放线菌LC-7,经初步鉴定为链霉菌属(Streptomyces sp.)。链霉菌LC-7的发酵液用氯仿、正丁醇萃取后,通过捷克八溶剂系统分析,判定该抗菌物质为碱性水溶性抗生素。抗菌物质进一步采用离子交换树脂纯化,并经醇沉后,获得抗生素的结晶,再用HPLC进一步纯化后并进行质谱分析可得出,LC-7所产抗菌活性物质为一种新的氨基糖苷类抗生素,其分子量为365.1。田间试验表明,链霉菌LC-7对烟草青枯病具有显著的防治效果,防效为85.48%。Tobacco bacterial wilt is a soil-borne disease caused by Ralstonia solanaceanm.A strain of Actinomycete LC-7with obvious antagonistic activities against R.solanaceanm was isolated from healthy soils and identified as Streptomyces sp.The fermentation broth of Streptomyces sp.LC-7was extracted with chloroform and n-butanol.The analysis through the Czech eight solvent systems revealed that the antibacterial substance was an alkaline water-soluble antibiotic.The antibacterial material was purified by exchange column,and precipitated with alcohol to obtain the crystal.The crystal was further purified by HPLC and analyzed by mass spectrometry.The results showed that the antimicrobial active substance was a new aminoglycoside antibiotic with a molecular weight of365.1.In the field experiment,Streptomyces sp.LC-7had significant control effect on tobacco bacterial wilt.The control efficacy was85.48%.

关 键 词：放线菌 青枯雷尔氏菌 氨基糖苷类抗生素 分离纯化 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]