有机胺对螺旋藻生长及固碳效果的影响  

Effects of Organic Amine on Spirulina Growth and Carbon Fixation

作　　者：王兆印[1] 李一锋[1] 张旭[1] 朱明龙[1] 谭文松[1] WANG Zhao-yin;LI Yi-feng;ZHANG Xu;ZHU Ming-long;TAN Wen-song(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology,Shanghai 200237, China)

机构地区：[1]生物反应器工程国家重点实验室,华东理工大学,上海200237

出　　处：《高校化学工程学报》2017年第2期377-386,共10页Journal of Chemical Engineering of Chinese Universities

摘　　要：采用微藻培养来降低空气中二氧化碳含量是当前研究的热点。然而,以气态CO_2为碳源的微藻培养过程中,普遍存在传质和固碳速率较低等问题。研究发现有机胺类物质如MEA(乙醇胺)、DEA(二乙醇胺)、MDEA(N-甲基-二乙醇胺)、TEA(三乙醇胺)等能够显著提高CO_2的气液传质速率。而针对有机胺类物质影响微藻培养及固碳效率的公开报道却并不多。研究以钝顶螺旋藻(FACHB-901)利用低含量CO_2气体为碳源的培养过程为对象,系统研究了有机胺类物质的种类(MDEA、MEA、DEA、TEA)及其添加策略对螺旋藻生物量和固碳速率的影响关系。研究结果表明,在改良的Zarrouk培养基中添加了1 mmol·L^(-1) MDEA可以使培养基中溶解性无机碳(DIC)含量达到297.4 mg·L^(-1)。而添加1mmol·L^(-1) TEA可以得到最佳的螺旋藻生物量(0.894 g·L^(-1))和固碳速率(139.3 mg·(L·d)^(-1))。同时,研究获得螺旋藻培养过程中TEA的最优添加策略为:延滞期添加5 mmol·L^(-1)、对数前期补加1 mmol·L^(-1)、对数后期补加2 mmol·L^(-1)。最终,通过TEA优化的添加策略获得了螺旋藻的生物量(1.248 g·L^(-1))和固碳速率(191.4 mg·(L·d)^(-1),比对照组分别提高了25.6%和41.2%。Research on biological CO2fixation with aquatic and marine microalgae has attracted great attention.However,when CO2(g)is the only carbon source in microalgae cultivation,there are problems such as low CO2mass transfer and carbon fixation rates.Research found that alkanolamine substances,such as MEA(an ethanol amine),DEA(diethanolamine),MDEA(N-methyl diethanolamine)and TEA(triethanolamine)significantly increase gas-liquid mass transfer rates of CO2into aqueous solution.However,three are fewstudies discussed effects of alkanolamine on microalgae cultivation and CO2fixation efficiency.This study investigated Spirulina platensis(FACHB-901)cultivation processes with low CO2(g)loading gas as the only carbon source.Effects of alkanolamine type(MDEA,MEA,DEA,and TEA)and alkanolamine adding strategy on biomass production and carbon fixation rates of Spirulina platensis were systematically studied.The results show that the soluble inorganic carbon(DIC)in the modified Zarrouk medium is297.4mg?L?1with the addition of1mmolL1MDEA,and the optimal biomass production(0.894gL1)and carbon fixation rate139.3mg(Ld)1are obtained via addition of1mmol?L?1TEA.Meanwhile,the optimal TEA adding strategy during the whole cultivation process is also obtained experimentally,which is5mmolL1TEA addition during delay phase,1mmolL1TEA at the earlier stage of logarithmic phase and2mmolL1TEA at the later stage of logarithmic phase.The final biomass amount of1.248gLand carbon fixation rate of191.4mg(Ld)?1are achieved,which are25.6%and41.2%higher than those of the control,respectively.

关 键 词：钝顶螺旋藻 有机胺类物质 优化策略 微藻培养 固碳 

分 类 号：X703[环境科学与工程—环境工程] X788