体外细胞培养低氧控制装置的研制  

Development of hypoxia control device for cell culture in vitro

作　　者：李荣荣[1] 赵彦文[1] 周军[1] 高美娟[1] 牛金城[1] 刘东旭[2] LI Rong-rong;ZHAO Yan-wen;ZHOU Jun;GAO Mei-juan;NIU Jin-cheng;LIU Dong-xu(Dezhou People's hospital, Dezhou 253000, China;The School of Stomatology in Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Rebuilding, Ji'nan 250012 China)

机构地区：[1]德州市人民医院,山东德州253000 [2]山东大学口腔医学院,山东省口腔组织再生重点实验室,山东济南250012

出　　处：《口腔疾病防治》2017年第5期323-326,共4页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金(81571010)

摘　　要：目的为更近似地模拟细胞在体内所处的低氧环境,研制一种低氧控制装置,以实现对体外细胞培养氧气浓度的自动化控制。方法体外细胞培养低氧控制装置由计算机控制的硬件和软件组成,硬件包括氧气控制与检测系统、氮气管路、细胞培养和抗干扰优化装置,软件是基于C语言的系统开发套件。当控氧仪经氧气传感器读取的氧气浓度值大于设定值时,氮气管路开放,氮气进入培养箱;当达到设定值时,氮气管路关闭,氮气不能通入,如此不断循环往复,从而使氧气浓度达到某一水平并维持稳定。通过将装置设定的氧气浓度与测氧仪测得的箱内实际氧气浓度进行比较及对氧气浓度达设定值后的波动情况进行监测,进一步验证装置的可行性。结果体外细胞培养低氧控制装置设定的氧气浓度与测得的箱内实际氧气浓度差异无统计学意义(P>0.05),且该装置对设定值模拟的精度可达±0.5%的要求,满足系统预定精度要求,并可保持较长时间稳定。结论本装置能有效控制氧气浓度达所需要求,能满足在低氧条件下进行体外细胞培养的要求。Objective To simulate the hypoxic environment of cells in vivo more approximately,a hypoxia control device which can control oxygen concentration automatically for cell culture in vitro was developed.Methods In vitro cell culture hypoxia control device consisted of hardware and software which were controlled by computer.The hardware was composed of oxygen detection and control system,nitrogen gas pipeline,cell culture and anti?jamming device,and the software was based on Clanguage.When oxygen controlle rreads the oxygen concent ration more than the set value,the nitrogen gas pipeline opens and nitrogen enter into the incubator;when reaching the set value,the nitrogen gas pipeline closes and nitrogen is not allowed to pass through.Circularly,a constant oxygen concentration was reached and maintained.This device was further verified by comparing the set value of the equipment with figures of the oxygen meter and by monitoring fluctuation condition after the oxygen concentration reaching the set value.Results There was no significant difference between the oxygen concentration set in the in vitro cell culture hypoxia control device and the actual oxygen concentration in the measured box(P>0.05),and the accuracy of the device to set the value of the simulation up to±0.5%of the requirements to meet the system accuracy requirements,and can remain stable for a long time.Conclusion The device can effectively control the oxygen concentration to the required requirements,to meet the conditions in the hypoxic conditions for in vitro cell culture.

关 键 词：低氧控制装置 细胞培养 氧气 氮气 

分 类 号：R318.6[医药卫生—生物医学工程]