南繁区稻瘟病菌遗传多样性和群体遗传结构的AFLP分析  

AFLP Analyses of Genetic Diversity and Population Genetic Structure of Magnaporthe oryzae from South China Crop Breeding Area

作　　者：朱名海 皮磊[1] 舒灿伟[1] 周而勋[1] ZHU Minghai;PI Lei;SHU Canwei;ZHOU Erxun(Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, China)

机构地区：[1]华南农业大学农学院广东省微生物信号与作物病害防控重点实验室,广州510642

出　　处：《中国水稻科学》2017年第3期320-326,共7页Chinese Journal of Rice Science

基　　金：国家公益性行业(农业)科研专项(201403075)

摘　　要：【目的】为了明确南繁区稻瘟病菌(Magnaporthe oryzae)的遗传分化情况,【方法】采用AFLP分子标记技术对南繁核心区(三亚、乐东和保亭)和非核心区(琼中、屯昌和定安)共60个稻瘟病菌菌株的遗传多样性和群体遗传结构进行了比较分析。【结果】聚类分析表明,几乎所有菌株都聚在同一个谱系里,并且该谱系没有明显的亚群;群体遗传结构分析表明,核心区群体的多态性位点百分率、Shannon信息指数和基因流分别为87.89%、0.2738和4.2897,高于非核心区群体的81.37%、0.2703和3.5892;然而,核心区群体的Nei基因多样性指数和基因分化系数分别为0.1657和0.1044,低于非核心区群体的0.1662和0.1223。【结论】这些结果表明核心区和非核心区菌株都存在丰富的遗传多样性,不同群体间均存在较多的基因交流,但遗传变异均主要来自群体内;相比之下,核心区菌株的遗传多样性和遗传分化程度较高。【Objective】In order to clarify the genetic differentiation of Magnaporthe oryzae in South China Crop Breeding Area in Hainan Province,【Method】the genetic diversity and population genetic structure of60Magnaporthe oryzae isolates collected from the core region(Sanya,Ledong and Baoting)and non-core region(Qiongzhong,Tunchang and Ding'an)of South China Crop Breeding Area were comparatively analyzed using amplified fragment length polymorphism(AFLP)technique.【Result】The cluster analysis showed that almost all isolates were clustered in one group,and there was no obvious subgroup.The analysis of population genetic structure showed that the percentage of polymorphic loci(PPL),Shannon's information index(I)and gene flow(Nm)in the core population were87.89%,0.2738and4.2897,respectively,higher than those of non-core population whose PPL,I and Nm were81.37%,0.2703and3.5892,respectively.However,Nei's gene diversity index(H)and genetic differentiation coefficient(Gst)in the core population were0.1657and0.1044,lower than those of non-core population whose H and Gst was0.1662and0.1223.【Conclusion】These results showed that there were rich genetic diversity in both isolates of core and non-core regions and wide gene flow existed among these populations,but the genetic variation was mainly within the population.In contrast,the genetic diversity and the degree of genetic differentiation in the isolates of core region were relatively larger.

关 键 词：南繁区 稻瘟病菌 遗传多样性 群体遗传结构 AFLP 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]