恩诺沙星对脊尾白虾APND、ECOD和GST活性的影响  

Effects of enrofloxacin on APND, ECOD and GST activities in Exopalaemon carinicauda

作　　者：翟倩倩[1,2] 李健[1,2] ZHAI Qian-qian;LI Jian(Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China)

机构地区：[1]中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室,山东青岛266071 [2]青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266200

出　　处：《海洋科学》2017年第2期53-60,共8页Marine Sciences

基　　金：山东省自然科学基金项目(BS2015HZ015);国家虾现代产业技术体系项目(CARS-47);山东泰山产业领军人才工程项目(LJNY2015002);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02)~~

摘　　要：为研究脊尾白虾(Exopalaemon carinicauda)药物代谢酶对恩诺沙星的响应及其在恩诺沙星代谢过程中的作用,作者以10、20和40 mg/kg恩诺沙星连续投喂脊尾白虾3 d,于投喂后的第1、2天以及停止投喂药物的第1、4、8、12、24、48、72、120小时取样,测定了脊尾白虾肝胰腺、鳃和血清中的氨基比林-N-脱甲基酶(aminopyrine-N-demethylase,APND)、7-乙氧基香豆素-O-脱乙基酶(7-ethoxycoumarinO-Deethylase,ECOD)和谷胱甘肽-S-转移酶(glutathione-S-transferase,GST)的活性。结果表明:不同浓度恩诺沙星对脊尾白虾肝胰腺、鳃和血清中Ⅰ相药物代谢酶APND和ECOD活性均呈现显著抑制作用(P<0.05)。投喂药物后,APND和ECOD活性呈现先下降后逐渐上升趋势,3个剂量组酶活性均在最后一次给药后8 h达到最低。与对照组相比,不同浓度恩诺沙星对各组织中Ⅱ相药物代谢酶GST活性呈现显著诱导作用(P<0.05),此作用呈现先上升后下降的趋势。恩诺沙星对脊尾白虾APND、ECOD及GST活性的作用均呈现剂量依赖性。本研究表明,脊尾白虾Ⅰ相和Ⅱ相代谢酶均会对药物做出反应,可作为其养殖过程中药物代谢及适用性的指示物。To study the response of drug metabolism enzymes to enrofloxacin and their function in the metabolism of enrofloxacin,shrimp(Exopalaemon carinicauda)were fed with medicated feed at doses of10,20,and40mg/(kg·BW)daily,and shrimp in each group were sampled on day1and day2during the drug exposure period and1,4,8,12,24,48,72,and120h after the end of the drug exposure period.Levels of aminopyrine-N-demethylase(APND),7-ethoxycoumarin-O-deethylase(ECOD),and glutathione-S-transferase(GST)were determined in the hepatopancreas,gills,and serum.Results showed that different concentrations of enrofloxacin could significantly inhibit the activities of phase I metabolism enzymes APND and ECOD in the hepatopancreas,gills,and serum(P<0.05).After feeding with the drug,the activities of APND and ECOD first decreased and then increased gradually.Activities of the metabolism enzymes in the three concentration groups were lowest at8h after dosing.Compared with the control group,different concentrations of enrofloxacin significantly induced the activities of phase II metabolism enzyme GST in the three tissues(P<0.05),and the induction of the activities were first increased and then decreased gradually.The effects of enrofloxacin on the activities of APND,ECOD,and GST were all dose dependent.In conclusion,this study demonstrated that phaseⅠand phaseⅡenzymes of E.carinicauda are sensitive parameters that could be used as biomarkers for evaluating the exposure to aquatic drugs.

关 键 词：脊尾白虾(Exopalaemon carinicauda) 恩诺沙星 氨基比林-N-脱甲基酶(APND) 7-乙氧基香豆素-O-脱乙基酶(ECOD) 谷胱甘肽-S-转移酶(GST) 

分 类 号：S948[农业科学—水产养殖] S913[农业科学—水产科学]