基于COI基因和D-loop区部分序列比较雷州半岛东、西岸湖栖鳍虾虎鱼群体的遗传多样性  

Genetic diversity of Gobiopterus lacustris populations from western and eastern Leizhou Peninsula based on partial sequences of mitochondrial COI gene and D-loop

作　　者：廖健[1] 龙水生 贺亮[1] 郭昱嵩[1] 刘楚吾[1] 刘丽[1] 王中铎[1] LIAO Jian;LONG Shui-sheng;HE Liang;GUO Yu-song;LIU Chu-wu;LIU Li;WANG Zhong-duo(Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China)

机构地区：[1]广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524025

出　　处：《海洋科学》2017年第2期103-110,共8页Marine Sciences

基　　金：国家自然科学基金项目(31201996);广东省高等学校优秀青年教师培养计划项目(Yq2013089);广东海洋大学优秀学位论文培育项目(201605)~~

摘　　要：为比较分析雷州半岛东、西两岸湖栖鳍虾虎鱼(Gobiopterus lacustris)遗传差异,以COI基因和D-loop区部分序列为分子标记,获取雷州半岛湖栖鳍虾虎鱼群体COI序列44条(九龙山群体21条,高桥群体23条)、D-loop序列53条(九龙山群体25条,高桥群体28条)。基于COI基因部分序列分析结果显示:619 bp的44条序列中,统计的变异位点29个;T、C、A、G碱基组成分别为:27.8%、31.0%、23.7%、17.5%,且A+T(51.5%)高于C+G(48.5%);单倍型多样性比较,九龙山群体(0.695)>高桥群体(0.324);遗传分化分析表明东、西群体分化显著(Snn=0.0.591,0.01<P<0.05),基因流(Nm)为5.37,遗传分化系数(Gst)为0.147,固定指数(Fst)为0.042(0.001<P<0.01),核苷酸差异数(Kxy)为2.547,核苷酸歧义度(Dxy)为0.004。而基于D-loop区部分序列分析结果表明:563 bp的53条序列中,变异位点133个;T、C、A、G碱基组成分别为:32.3%、14.8%、35.5%、17.4%,A+T(67.8%)明显高于C+G(32.2%);单倍型多样性与COI分析结果类似,九龙山群体(0.797)>高桥群体(0.661);遗传分化分析表明东、西群体分化极显著(Snn=0.970,P<0.001),Fst为0.315(P<0.001),Nm值为0.55,Gst、Kxy、Dxy分别为:0.157、3.506、0.006;AMOVA分析揭示群体内变异程度高于群体间;聚类分析表明,基于COI基因与D-loop区部分序列构建的邻位链接法聚类树(NJ树)均存在按照采样点聚类的现象,但D-loop更为明显。综上,雷州半岛东、西两岸湖栖鳍虾虎鱼群体出现较为明显的分化现象。We compared two Gobiopterus lacustris populations collected from the eastern and western Leizhou Peninsula based on COI gene and D-loop region.The partial sequences of COI gene from44individuals(21from eastern Jiulongshan population and23from western Gaoqiao population)and D-loop region from53individuals(25from JLS and28from GQ)were cloned.Based on COI gene,the results were as follows:there were29variable sites in619bp COI sequences.The frequencies of T,C,A,and G were27.8%,31.0%,23.7%,and17.5%,respectively,with more A+T(51.5%)than C+G(48.5%).The haplotype diversity of Jiulongshan population(0.695)was higher than that of Gaoqiao population(0.324).Genetic differentiation analysis indicated that the eastern and western populations were differentiated significantly(Snn=0.591,0.01<P<0.05).The correlative values of Nm,Gst,Fst,Kxy,and Dxy were5.37,0.147,0.042,2.547,and0.004,respectively.However,much more variable sites(133bp)were observed in D-loop regions with563bp length.In addition,there were higher frequencies of A+T(67.8%)than COI,and the haplotype diversity of Jiulongshan population(0.797)was higher than that of Gaoqiao population(0.661).Genetic differentiation of the eastern and western populations was highly significant(Snn=0.970,P<0.001).The values of Nm,Gst,Fst,Kxy,and Dxy were0.157,0.315,3.506,and0.006,respectively.Analysis of molecular variance revealed that the percentage of variation within populations was higher than that among populations.Cluster analysis showed the presence of clustering phenomenon,which gathered by the collected sites of sample based on COI gene and D-loop region,but D-loops were more apparent.In summary,a significant differentiation occurred between eastern and western Leizhou Peninsula.

关 键 词：COI D-LOOP 湖栖鳍虾虎鱼(Gobiopterus lacustris) 雷州半岛群体 遗传分化 

分 类 号：S917[农业科学—水产科学]