海水中致病菌检测研究进展  

Research progress in detecting pathogenic bacteria in seawater

作　　者：刘淑文[1,2] 丁家旺[1] 秦伟[1] LIU Shu-wen;DING Jia-wang;QIN Wei(Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, China;University of Chinese Academy of Sciences, Beijng 100049, China)

机构地区：[1]中国科学院海岸带环境过程与生态修复重点实验室(烟台海岸带研究所),山东省海岸带环境过程重点实验室,中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049

出　　处：《海洋科学》2017年第2期145-156,共12页Marine Sciences

基　　金：中国科学院战略性先导科技专项(XDA11020702);山东省泰山学者人才计划项目(TS20081159)~~

摘　　要：海水中致病菌种类繁多、致病性强,对水产养殖和人体健康存在着潜在危害。传统的检测方法培养过程复杂繁琐、耗时长、效率低,难以满足实际工作的需求。因此,建立海水中致病菌的快速、灵敏、准确的检测技术势在必行。本文综述了流式细胞技术、分子生物学方法、免疫学方法和生物传感器技术等在海水致病菌检测中的应用,总结了上述方法在海水致病菌检测中的研究现状。There are many different kinds of pathogenic bacteria in seawater,each of which are potentially hazardous to aquaculture and human health.Conventional methods for detecting pathogenic bacteria have the drawbacks of complicated culturing procedures,time-consuming labor,and low efficiency,which limits their practical application.Therefore,there is an urgent need for the development of rapid,sensitive,and accurate detection technology for pathogenic bacteria in seawater.In this paper,we provide an overview of the applications of flow cytometry,the molecular biology method,the immunology method,and biosensors with respect to bacteria in seawater and summarize the research status of these methods.

关 键 词：海水致病菌 检测 流式细胞技术 分子生物学方法 免疫学方法 生物传感器技术 

分 类 号：P736.22[天文地球—海洋地质]