辽东楤木叶总皂苷对人结肠癌HT-29细胞凋亡的影响  

Effects of ETS on Apoptosis of Human Colorectal Cancer HT-29 Cells

作　　者：尹丽颖[1] 李凤金[1] 仲丽丽[1] 敖鹏[1] 张妍妍[1] 边晓燕[1] 唐丹丽[2] YIN Li-ying;LI Feng-jin;ZHONG Li-li;AO Peng;ZHANG Yan-yan;BIAN Xiao-yan;TANG Dan-li(Heilongjiang University of Chinese Medicine, Harbin 150040, China;China Academy of Chinese Medical Sciense, Beijing 100700, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]中国中医科学院,北京100700

出　　处：《中国中医药信息杂志》2017年第6期49-52,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：黑龙江中医药大学优秀创新人才支持计划项目(051226);黑龙江中医药大学科研基金项目(201218);哈尔滨市科技局项目(404S223)

摘　　要：目的探讨辽东楤木叶总皂苷(ETS)诱导人结肠癌HT-29细胞凋亡及其对凋亡相关蛋白表达的影响。方法 MTT法检测不同浓度(6.25、12.5、25、50、100、200 mg/kg)ETS培养的结肠癌HT-29细胞增殖率,Hoechst33258染色和激光共聚焦成像检测细胞凋亡,并观察凋亡细胞的形态学改变,免疫组化检测凋亡相关蛋白Bcl-2、Bax的表达。结果 ETS可诱导HT-29细胞发生凋亡,且凋亡作用在一定范围内呈剂量依赖性;ETS可使HT-29细胞Bcl-2表达降低、Bax表达升高(P<0.05,P<0.01),且呈明显的量效关系。结论 ETS可诱导HT-29细胞凋亡,其凋亡机制可能与降低Bcl-2、升高Bax的表达相关。Objective To study the apoptosis of human colorectal cancer HT-29cells induced by Aralia elataSeem leaf total saponin(ETS)and its effects on the expression of relevant proteins.Methods MTT assay was used todetect the proliferation of human colorectal cancer HT-29cells cultivated with different concentrations(6.25,12.5,25,50,100,200mg/kg)of ETS.Hoechst33258staining and laser confocal imaging were used to detect the apoptoticcells.Morphological changes were observed.The expressions of Bcl-2and Bax were detected by immunohistochemistry.Results ETS could induce apoptosis of HT-29cells and apoptosis was in a dose-dependent manner ina certain range.ETS could decrease the expression of Bcl-2and increase the expression of Bax in HT-29cells(P<0.05,P<0.01),showing a significant dose-effect relationship.Conclusion ETS can induce the apoptosis of HT-29cells,and the mechanism may be related to reducing the expression of Bcl-2and increasing the expression of Bax.

关 键 词：辽东楤木叶总皂苷 HT-29细胞 细胞凋亡 Bcl-2 BAX 

分 类 号：R285.5[医药卫生—中药学]