电针对坐骨神经损伤大鼠脑源性神经营养因子的影响  

Effects of Electroacupuncture on Brain Derived Neurotrophic Factor of Rats with Sciatic Nerve Injury

作　　者：叶晓春[1] 邵水金[1] 国海东[1] 韩小晶[1] 刘玉璞[1] 陆萍萍[1] YE Xiao-chun;SHAO Shui-jin;GUO Hai-dong;HAN Xiao-jing;LIU Yu-pu;LU Ping-ping(School of Basic Medicine, Shanghai University of TCM, Shanghai 201203, China)

机构地区：[1]上海中医药大学基础医学院,上海201203

出　　处：《中国中医药信息杂志》2017年第6期60-63,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81373754)

摘　　要：目的观察电针对坐骨神经损伤(SNI)大鼠脑源性神经营养因子(BDNF)的影响,探讨其治疗SNI的生物学机制。方法选取成年雄性Wistar大鼠50只,分离并切断大鼠坐骨神经后于体式显微镜下将两侧断端拉入神经再生室内造模。大鼠随机分为正常组、假手术组、模型组和电针组。电针组进行电针治疗,连续28 d。治疗结束后,HE染色观察神经再生,免疫荧光检测神经组织和脊髓BDNF的表达,ELISA检测血清BDNF的表达。结果电针组轴突再生数量明显多于模型组,且大体轮廓较清晰;与模型组比较,电针组可促进大鼠神经组织、脊髓和血清BDNF的表达(P<0.01)。结论电针可能通过上调神经损伤后BDNF的表达,促进大鼠SNI的再生修复。Objective To explore the effects of electroacupuncture on brain derived neurotrophic factor(BDNF)of rats with sciatic nerve injury(SNI);To discuss its biological mechanism for treatment of SNI.Methods Fifty adultmale Wistar rats were chosen,and the sciatic nerves of rats were cut off and pulled on both sides of the cut ends intonerve regeneration chamber.The rats were randomly divided into normal group,sham-operation group,model group,and electroacupuncture group.In the electroacupuncture group,the rats were treated by electroacupuncture for28days.After the treatment,the nerve regeneration was observed through HE staining.Immunofluorescence was used toanalyze the expression changes of BDNF in the nerve tissue and spinal cord.ELISA was used to observe the changesof expression of serum BDNF.Results The amount of axon regeneration in the electroacupuncture group wasobviously more than that in the model group,and the outline of the tissue more clear.Electroacupuncture couldpromote the expression of BDNF in the nerve,spinal cord and serum of SNI of rats compared with model group(P<0.01).Conclusion Electroacupuncture can promote the repairment and regeneration of SNI in rats by upregulatingthe expression of BDNF.

关 键 词：电针 坐骨神经损伤 脑源性神经营养因子 轴突 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]