基于ITS2条形码和化学指纹鉴别何首乌及其伪品  

Identification of Polygonum multiflora and Its Adulterants based on ITS2 Barcode and Chemical Fingerprint

作　　者：尚飞能 方海兰 段宝忠[1,2] Shang Feineng;Fang Hailan;Duan Baozhong(College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, Beijing 100071, China)

机构地区：[1]大理大学药学与化学学院,云南大理671000 [2]中国中医科学院中药研究所,北京100071

出　　处：《大理大学学报》2017年第4期12-15,共4页Journal of Dali University

基　　金：国家自然科学基金资助项目(31460084);云南省中青年学术技术带头人后备人才项目(2015HB058);云南省教育厅科学研究基金资助项目(2014Z135)

摘　　要：目的:研究何首乌及其伪品的分子和化学鉴别方法,为其用药安全提供保障。方法:采用DNA条形码和高效液相色谱法,比较何首乌及其伪品酱头ITS2条形码和HPLC化学指纹图谱的差异。结果:何首乌及酱头ITS2长度分别为193 bp和206bp,何首乌种内K2P遗传距离小于酱头的种间距离,且两者化学指纹存在明显差异。结论:DNA条形码和化学指纹图谱方法,是鉴别何首乌及伪品的有效手段。Objective:To develop a rapid identification method to discriminate Polygonum multiflorum and its adulterants,and to guarantee the quality and safety in medication.Methods:DNA barcode and high performance liquid chromatography(HPLC)were used to compare the ITS2barcode and the HPLC chemical fingerprint between Polygonum multiflorum and Polygonum denticulatum.Results:The ITS2length of Polygonum multiflorum and Polygonum denticulatum were193bp and206bp,respectively.The intraspectific K2P genetic distance of Polygonum multiflorum was shorter than the intraspectific distance of Polygonum denticulatum.Furthermore,there were obvious differences between their chemical fingerprints.Conclusion:DNA barcode and chemical fingerprint both can be used to identify Polygonum multiflorum and its adulterants effectively.

关 键 词：何首乌 伪品 DNA条形码 化学指纹 ITS2 

分 类 号：R282.5[医药卫生—中药学]