离子色谱安培法测定尿液中的胱氨酸  

Determination of Cystine in Urine by Ion Chromatography with Pulsed Amperometric Detection

作　　者：石磊[1] 钟春明 林青峰 刘慧 潘广文 李仁勇 Shi Lei;Zhong Chunming;Lin Qingfeng;Liu Hui;Pan Guangwen;Li Renyong(Yantai Yuhuangding Hospital, Yantai　264000, China;The Criminal Investigation Branch of Longyan Municipal Public Security Bureau, Longyan　364000, China;Quanzhou Environmental Monitoring Station, Quanzhou　362001, China;ThermoFisher Scenitific, Shanghai　201206, China)

机构地区：[1]烟台毓璜顶医院,山东烟台264000 [2]龙岩市公安局刑侦支队,福建龙岩364000 [3]泉州市环境监测站,福建泉州362001 [4]赛默飞世尔科技(中国)有限公司,上海201206

出　　处：《化学分析计量》2017年第3期72-75,共4页Chemical Analysis And Meterage

摘　　要：建立离子色谱安培法检测尿液中胱氨酸的分析方法。将尿液稀释处理后,过OnGuardⅡRP前处理柱,去除尿液中的有机物,选用低容量阳离子交换柱IonPac CS17进行分离,以甲烷磺酸梯度淋洗,柱后用300 mmol/L NaOH溶液衍生,离子色谱脉冲安培法检测,外标法定量。胱氨酸的质量浓度在0.1~5.0 mg/L范围内与其色谱峰面积呈良好的线性,线性相关系数r^2=0.999 9,检出限为0.05 mg/L。测定结果的相对标准偏差为0.71%(n=6),样品加标回收率为94%~108%。该方法具有操作简单,专属性强,灵敏度高等优点,可用于临床快速检测尿液中的胱氨酸。The method for the determination of cystine in urine was developed by ion chromatography with pulsed amperometric detection.The urine sample was diluted,then the organic matter in the urine was removed by a OnGuardⅡRP pretreatment column,and the filter liquor was separated by using low capacity of cation exchange column IonPac CS17with methane sulfonic acid as gradient elution,and derived by300mmol/L NaOH solution,the content of cystine in urine was determined with ion chromatography pulse ampere method and external standard method.The mass concentration of cystine in urine had good linear relationship with the chromatography peak area in the range of0.1-5.0mg/L with the correlation coefficients(r2)of0.9999.The detection limit of the method was0.05mg/L.The relative standard deviation of determination results was0.71%(n=6).The recovery of sample was94%-108%.The method is suitable for the determination of cystine in urine with a simple operation,high specificity and sensitivity.

关 键 词：胱氨酸 尿液 安培检测 离子色谱法 

分 类 号：O657.7[理学—分析化学]