一种新颖深海微生物羧酸酯酶制备(R)-苯乙醇的研究  

Enantioselective production of (R)-1-phenylethanol by a novel marine microbial carboxylesterase

作　　者：黄锦龙[1,2] 张云[1,3] 孙爱君[1,3] 胡云峰[1,3,4] HUANG Jinlong;ZHANG Yun;SUN Aijun;HU Yunfeng(Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China;University of Chinese Academy of Sciences, Beijing 100049, China;Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences,Guangzhou, Guangdong 510301, China;South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, Guangdong 510275,China)

机构地区：[1]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东广州510301 [2]中国科学院大学,北京100049 [3]中国科学院南海海洋研究所,广东省海洋药物重点实验室,广东广州510301 [4]南海生物资源开发和利用协同创新中心,广东广州510275

出　　处：《热带海洋学报》2017年第3期55-60,共6页Journal of Tropical Oceanography

基　　金：中国科学院战略性先导科技专项(XDA11030404);广州市科技计划项目(201510010012);国家自然科学基金青年科学基金项目(21302199)~~

摘　　要：深海微生物酯酶BSE00077能够有效地水解拆分消旋乙酸苏合香酯,制备(R)-1-苯乙醇。优化的反应条件为50mmol×L^(-1)底物,10%体积分数的正庚烷作为溶剂,在p H 7.0和30℃条件下反应2h。经过优化后,目标产物(R)-1-苯乙醇的对映体过量值超过99%,转化率超过30%。我们发现酯酶BSE00077也能够拆分不同酰基链长的1-苯乙醇酯,酰基链长会影响1-苯乙醇的对映体过量值。深海来源的酯酶BSE00077作为生物催化剂在工业上制备(R)-1-苯乙醇和其他手性化工产品具有很好的应用潜力。Deep-sea microbial esterase BSE00077can efficiently resolve(±)-1-phenylethyl acetate and generate(R)-1-phenylethanol.The optimal working conditions for the enzymatic resolution of(±)-1-phenylethyl acetate by marine microbialesterase BSE00077were found to be:50mmol/L(±)-1-phenylethyl acetate,10%n-heptane(v/v),pH7.0,30°C for2h.Afterprocess optimization,desired optically pure product(R)-1-phenylethanol was obtained with an enantiomeric excess of over99%and a conversion of over30%.Esterase BSE00077can also enzymatically resolve some other racemic1-phenylethylesters with different chain lengths.We found that the chain lengths could greatly affect the enantiomeric excess of desiredproduct.Esterase BSE00077is a novel marine microbial esterase with great potential in the asymmetric synthesis of(R)-1-phenylethanol as well as of other chiral chemicals in industry.

关 键 词：深海微生物酯酶 (R)-1-苯乙醇 水解反应 动力学拆分 

分 类 号：P735.4[天文地球—海洋生物学]