Transformation of iron in pure culture process of extremely acidophilic microorganisms  

极端嗜酸微生物纯培养过程中铁元素的转化（英文）

作　　者：Jing-hua FANG Yong LIU Wan-li HE Wen-qing QIN Guan-zhou QIU Jun WANG 方京华;刘咏;何万里;覃文庆;邱冠周;王军(中南大学粉末冶金国家重点实验室,长沙410083;中南大学资源加工与生物工程学院,长沙410083;中南大学生物冶金教育部重点实验室,长沙410083)

机构地区：[1]State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China [2]School of Minerals Processing & Bioengineering, Central South University, Changsha 410083, China [3]Key Laboratory of Biohydrometallurgy, Ministry of Education, Central South University, Changsha 410083, China

出　　处：《Transactions of Nonferrous Metals Society of China》2017年第5期1150-1155,共6页中国有色金属学报（英文版）

基　　金：Project(51374248)supported by the National Natural Science Foundation of China;Project(NCET-13-0595)supported by the Program for New Century Excellent Talents in University of China;Project(2016-SSRF-PT-006152)supported by the Shanghai Synchrotron Radiation Facility(SSRF),China;Project(2016-BEPC-PT-000855)supported by the Beijing Synchrotron Radiation Facility(BSRF),China

摘　　要：Jarosite and extracellular polymer substance generated during pure culture and bioleaching process have been widely accepted the main transformation of decreasing iron in the medium.In the present work,acidophilus bioleaching organisms Ferroplasma thermophilum,Leptospirillum ferriphilum and Acidithioobacillus ferrooxidans were cultured.It was found that they can live in low pH environment,and more than10particles in each cell intracellular nano-particles are synthesized in the cells.By analyzing the morphology and chemical composition of nano-particles,they were found to contain iron,and the three microorganisms belonged to high-yielding strains.The results show that the transformation of the decreasing iron ions is not only generating jarosite,but also taken into cells and synthesizing ferruginous nano-particles.在培养浸矿微生物过程中,培养基中的铁含量不断减少,一般认为主要是生成了黄钾铁矾沉淀与胞外多聚物。对三种嗜酸浸矿菌Ferroplasma thermophilum,Leptospirillum ferriphilum和Acidithioobacillus ferrooxidans进行培养,发现他们能在嗜酸环境下生存,而且平均每个细胞生成超过10个纳米颗粒。通过分析纳米颗粒的形态与成分,发现颗粒中含有铁,而且纳米颗粒的产量很高。结果表明,在纯培养过程中减少的铁不仅参与生成黄钾铁矾,同时也被吸收进入细胞,合成含铁的纳米颗粒。

关 键 词：JAROSITE extracellular polymer substance BIOLEACHING ferruginous nano-particles 

分 类 号：TF18[冶金工程—冶金物理化学]