烟草花叶病毒山西番茄分离物的全基因组序列测定及分析  

Complete Whole Genome Sequence Measure and Analysis of Tobacco mosaic virus Infecting Tomato in Shanxi

作　　者：庞小静[1] 赵慧琪 王德富[1] 刘勇[2] 牛颜冰[1] PANG Xiao-jing;ZHAO Hui-qi;WANG De-fu;LIU Yong;NIU Yan-bing(College of Life Sciences,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Plant Protection Institute Hunan Academy of Agricultural Science,Changsha 410125,Hunan,China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]湖南省农业科学院植物保护研究所,湖南长沙410125

出　　处：《中国蔬菜》2017年第6期50-55,共6页China Vegetables

基　　金：公益性行业(农业)科研专项(201303028);国家自然科学基金项目(31540050)

摘　　要：为明确山西省晋中市侵染番茄的病毒种类,采用双链RNA(double-stranded RNA,ds RNA)技术和非序列依赖PCR扩增(sequence-independent amplification,SIA)方法对感病番茄进行分子鉴定。序列测定及分析发现,具有花叶、卷曲症状的番茄为烟草花叶病毒(Tobacco mosaic virus,TMV)所侵染。进一步克隆TMV番茄分离物(TMV-SXFQ)的全基因组序列,得到TMV-SXFQ(Gen Bank登录号为JX993906)全长为6 394 bp,含4个开放阅读框(open reading frame,ORF)。序列比对分析表明,TMV-SXFQ与烟草花叶病毒属其他分离物的同源性为86.3%~99.6%;系统进化分析表明,TMV-SXFQ与韩国分离物IM聚为一簇、亲缘关系最近。To identify the virus pathogen inducing tomato(Solanum lycopersicum L.)mosaic and curl disease at Jinzhong City of Shanxi Province,the technology of double-stranded RNA(dsRNA)and sequenceindependent amplification(SIA)were used.Results of SIA and sequencing showed that there was Tobacco mosaic virus(TMV)in affected tomato leaves.Further cloning the whole genome sequence of TMV(TMVSXFQ),the TMV-SXFQ was gained(GenBank No.JX993906),which was6394bp in length and presented a typical Tobamovirus genome organization with4ORFs.Sequence comparative analysis showed that the homology of TMV-SXFQ and Tobacco mosaic virus and other isolates was86.3%-99.6%.Phylogenetic analysis suggested that TMV-SXFQ isolates had closeer ties consanguinity with IM strain from South Korea and these2isolates formed an independent branch.

关 键 词：番茄 病原鉴定 烟草花叶病毒 序列分析 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]