云南省部分活禽零售市场休市前后禽流感病毒动态监测  

Dynamic Monitoring of Avian Influenza Virus before and after the Closure of Some Live Poultry Retail Markets in Yunnan Province

作　　者：田康[1,2] 王镇 赵焕云[2] 杨东 李国云 段博芳[2] 张应国[2] 范泉水[5] 李莲军[1] 张文东[2] 张富强[5] Tian Kang;Wang Zhen;Zhao Huanyun;Yang Dong;Li Guoyun;Duan Bofang;Zhang Yingguo;Fan Quanshui;Li Lianjun;Zhang Wendong;Zhang Fuqiang(Yunnan Agriculture University,Kunming,Yunnan　650223;Centre for Animal Disease Control and Prevention of Yunnan Province,Kunming,Yunnan　650201;Centre for Animal Disease Control and Prevention of Diqing Municipality,Shangri-La,Yunnan　674400;Centre for Animal Disease Control and Prevention of Shangri-La City,Shangri-La,Yunnan　674400;Centre for Disease Control and Prevention of Chengdu Military Region,Kunming,Yunnan　650118)

机构地区：[1]云南农业大学,云南昆明650223 [2]云南省动物疫病预防控制中心,云南昆明650201 [3]迪庆州动物疫病预防控制中心,云南香格里拉674400 [4]香格里拉市动物疫病预防控制中心,云南香格里拉674400 [5]成都军区疾病预防控制中心,云南昆明650118

出　　处：《中国动物检疫》2017年第6期1-5,共5页China Animal Health Inspection

基　　金：国家公益性行业专项(201103008);军队科技计划项目(BWS14J025)

摘　　要：[目的]监测活禽零售市场休市前后家禽和环境中禽流感病毒的分布和波动状况,评估定期休市干预效果。[方法]在2015年9月28日至11月11日的3轮休市期间,从云南省选取4个有代表性的活禽零售市场,于休市的前1天、休市当日及休市后1天(复市当日)、3天、5天、10天,采集家禽喉气管、咽喉/泄殖腔棉拭子和环境样品,连续进行3轮采样,采用甲型流感病毒通用型RT-PCR方法,检测样品中的病毒核酸,开展禽流感病毒分布动态监测和休市干预效果评估。[结果]不同市场、不同休市轮次的休市干预效果参差不齐;禽流感病毒在鸡鸭混杂销售的市场中呈现持续分布,而在仅有鸡销售的市场中呈间断或零星分布;鸭群样品阳性率高于环境样品和鸡群样品阳性率。[结论]研究结果提示:鸭群对活禽零售市场的禽流感病毒传入、扩散和持续分布发挥着极其重要的作用;活禽零售市场的休市干预效果受多种因素影响,只有科学规范实施休市制度和严格执行监管措施,才能获得良好的休市干预效果。[Objective]To monitor the distribution and fluctuation of avian influenza virus in poultry and environment before and after the closure of live poultry markets,and to evaluate the effect of regular intervention.[Methods]Samples including poultry larynx and trachea tissues,throat/cloaca and environmental cotton swabs were collected from four representative markets on the first day before closure,the closing day and the first,the third,the fifth and the tenth day after closure in three consecutive rounds during28th September to11th November in2015.The viral nucleic acids of the samples were detected using influenza type A virus universal RT-PCR for surveillance of avian influenza virus dynamics and evaluation the intervention effect.[Results]The results showed that the effectiveness of closure intervention among the different markets and rounds were uneven or varied.The distribution of avian influenza virus in the chickens and ducks mixed markets was persistent and continuous,but discontinuous or sporadic in the only chicken sales markets.The positive rate of viral nucleic acids in ducks was higher than in chickens or environment.[Conclusion]The results suggested that the duck played a very important role in introduction,transmission,spreading,and continuous distribution of avian influenza virus in live poultry retail markets.The intervention effectiveness of market closure were influenced or disturbed by many factors.To obtain good intervention effect,the regulations of market closure and supervision should be implemented scientifically,standardly and strictly.

关 键 词：禽流感病毒 活禽市场 休市 监测 

分 类 号：S852.659.5[农业科学—基础兽医学]