参苓白术散对CD大鼠血清及结肠组织中IL-1β、IL-4及IL-10含量的影响  

Effects of Shenling Baizhu San on IL-1β, IL-4 and IL-10 contents in colon serum and tissue of rats with Crohn's disease

作　　者：余焕钦 贾育新[1,2] 

机构地区：[1]甘肃中医药大学,甘肃兰州730020 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730020

出　　处：《中医临床研究》2017年第11期4-6,共3页Clinical Journal Of Chinese Medicine

摘　　要：目的:通过观察参苓白术散对克罗恩氏病(CD)大鼠血清及组织中白介素1β、白细胞介素4(IL-4)及白细胞介素10(IL-10)含量的影响,探讨参苓白术散治疗克罗恩氏病的效应机制。方法:受试大鼠按随机数字表法分为空白组和模型组,采用三硝基苯磺酸(TNBS)合乙醇溶液灌肠制备克罗恩氏病大鼠模型。造模成功后将模型大鼠分为模型组、参苓白术散组和阳性对照组(美沙拉嗪组),空白组和模型组大鼠每日给予10ml/kg蒸馏水灌胃,参苓白术散组给予12g·kg-1·d-1参苓白术散药剂灌胃,阳性对照组给予0.2g·kg-1·d-1美沙拉嗪颗粒灌胃,持续治疗21d后,分别对各组大鼠血清及组织中IL-1β、IL-4和IL-10含量进行检测。结果:与空白组比较,模型组大鼠一般生存状况较差,血清及组织中IL-1β含量显著升高,IL-4和IL-10含量降低(P<0.05);与模型组比较,参苓白术散组大鼠一般生存状况明显改善,血清及结肠组织中IL-1β含量显著降低(P<0.05),而IL-4和IL-10含量升高(P<0.05)。结论:参苓白术散具有改善克罗恩氏病大鼠一般生存状况,调节结肠组织Th1/Th2细胞因子平衡分泌的作用。Objective:To study effects of Shenling Baizhu San(SLBZ)on IL-1β,IL-4and IL-10contents in colon serum and tissueof rats with Crohn's disease.Methods:Rats were randomly divided into2groups:normal group and model group.After UC modle wassuccessful,UC rats were numbered and grouped by random number table into the model group,SLBZ group and positive group;then ratsin normal and model group were intragastrically administrated with distilled water10ml/kg every day,SLBZ group with SLBZ volumeof12g?kg-1?d-1,and positive group with suspension of mesalazine0.2g?kg-1?d-1,for21consecutive days.Levels of IL-1β,IL-4,and IL-10in colon serum and tissue of rats in each group were detected.Results:In successful model group,generally living conditions of ratswere poor,levels of IL-1βin colon serum and tissue were much higher(P<0.01);but levels of IL-4and IL-10were lower than thosein the normal group(P<0.05).Compared with the model group,the general survival of the state in the SLBZ San group was improvedsignificantly.SLBZ could increase levels of IL-4and IL-10significantly(P<0.05),decrease levels of IL-1β(P<0.05).Conclusion:It wassuggested that SLBZ has functions of improving general living conditions,adjusting cytokine balance of TH1/TH2.

关 键 词：白细胞介素1Β 白细胞介素4 白细胞介素10 克罗恩氏病 参苓白术散 

分 类 号：R285[医药卫生—中药学]