兔口腔黏膜上皮细胞与PCL电纺纤维的复合尿道支架的制备及可行性分析  

The preparation and feasibility study of composite urethral stent-rabbit oral mucosal epithelial cells and PCL electrospun fiber

作　　者：关勇[1] 孟庆娅[1] 徐国栋[1] 张富义[1] 舒剑波[1] GUAN Yong;MENG Qing-ya;XU Guo-dong;ZHANG Fu-yi;SHU Jian-bo(Department of Urology, Tianjin Children’s Hospital, Tianjin 300134, Chi)

机构地区：[1]天津市儿童医院泌尿外科,300134

出　　处：《天津医药》2017年第6期584-587,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金重点项目(2013KR08)

摘　　要：目的探讨兔口腔黏膜上皮细胞与聚己内酯(PCL)电纺纤维的复合支架用于尿道修复的可行性。方法用三氯甲烷和无水甲醇体积比5∶1的混合液配制25%PCL混合液,通过静电纺丝得到PCL纤维管状支架,在其管腔内种植1.5×105个兔口腔黏膜上皮细胞,然后埋入裸鼠皮下,2周后取出,通过HE和细胞角蛋白抗体免疫荧光染色对该复合尿道支架进行生物学评价。未种植兔口腔黏膜上皮细胞的PCL管为对照组,其评价方法同上。结果与对照组比较,种植兔口腔黏膜上皮细胞的PCL管展现出良好的细胞化,并且兔口腔黏膜上皮细胞在PCL管腔表面形成了一层致密的细胞层,兔口腔黏膜上皮细胞能够在PCL管腔表面存活增殖。结论兔口腔黏膜上皮细胞可以作为组织工程化尿道支架的种子细胞来源之一,其与PCL电纺纤维相结合,可以构建适合于尿道修复需要的组织工程替代材料。Objective To explore the feasibility of using composite scaffolds of rabbit oral epithelial cells andpolycaprolactone(PCL)electrospun fibers for urethral repair.Methods The25%PCL was prepared using a5∶1by volumemixture of trichloromethane and anhydrous methanol,and PCL fiber tubular scaffolds were obtained by electrospinning.Rabbit oral mucosa epithelial cells(1.5×105)were implanted on the PCL scaffold.Subsequently,they were embedded innude mice subcutaneous,explanted in2weeks.PCL fiber tubular scaffolds without rabbit oral mucosa epithelial cells wereused as control.The complex urethral scaffolds were evaluated by immunofluorescence staining with cytokeratin antibodyand HE staining.Results Compared with blank PCL group,the rabbit oral mucosa epithelial cell group showed a goodcellularization.Rabbit oral mucosa epithelial cells formed a dense cell layer on the surface of PCL lumen,which suggestedthat rabbit oral mucosa epithelial cells can proliferate on the surface of PCL lumen.Conclusion Rabbit oral epithelial cellscan be used as one of the seed cells for tissue engineered urethral scaffolds,and it is possible to construct tissue engineeringsubstitute materials for urethral repair by rabbit oral epithelial cells combined with PCL.

关 键 词：口腔黏膜 上皮细胞 组织工程 聚己内酯 尿道支架 静电纺丝 

分 类 号：R695[医药卫生—泌尿科学]