普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定  

Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species

作　　者：董雪[1] 刘梦[1] 赵献林[2] 冯玉梅[1] 杨燕[1] DONG Xue;LIU Meng;ZHAO Xian-Lin;FENG Yu-Mei;YANG Yan(The functional Laboratory of Plant Biotechnology, College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China;Wheat Research Institute, Henan Academy of Agricultural Sciences / Henan Key Laboratory of Wheat Biology / National Engineering Laboratory for Wheat / Key Laboratory of Wheat Biology and Genetic Breeding in Central Huang-Huai Region, Ministry of Agriculture, Zhengzhou 450002, China)

机构地区：[1]内蒙古农业大学生命科学学院植物生物技术功能实验室,内蒙古呼和浩特010018 [2]河南省农业科学院小麦研究所/河南省小麦生物学重点实验室/小麦国家工程实验室/农业部黄淮中部小麦生物学与遗传育种重点实验室,河南郑州450002

出　　处：《作物学报》2017年第6期829-838,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31271726);内蒙古自治区自然科学基金项目(2014MS0338);内蒙古农业大学优秀青年科学基金项目(2014XYQ-18);教育部春晖计划项目(Z2009-1-01060)资助~~

摘　　要：低分子量麦谷蛋白约占小麦种子贮藏蛋白的三分之一,对面团延展性和食品加工品质有重要影响。普通小麦近缘种是小麦遗传改良的重要基因资源。本研究利用Glu-A3位点特异性引物对野生二粒小麦、栽培二粒小麦、硬粒小麦及野生一粒小麦共计9份材料进行Glu A3-1、Glu A3-2、Glu A3-3基因扩增和鉴定,各发现5个等位变异,共计15个单元型;其中,有2个等位变异含有9个半胱氨酸残基,可能属于优良品质亚基。对小麦近缘种中这些Glu-A3位点等位变异的鉴别,进一步完善了小麦低分子量麦谷蛋白亚基的构成,并为小麦品质育种中亲本的选择提供了相应依据。Low-molecular-weight glutenin subunits(LMW-GS)account for about one-third of wheat seed storage proteins andhave great effects on dough extensibility and food processing quality.Common wheat relative species are important genetic resourcesfor wheat improvement.In this study,the specific primers of Glu-A3genes were used to identify the allelic variations ofGluA3-1,GluA-2,and GluA3-3from nine accessions of Triticum dicoccoides,T.dicoccum,T.durum,and T.boeoricum.A total of15new haplotypes were identified,including five in GluA3-1,five in GluA3-2,and five in GluA3-3.Among them,two haplotypeseach contained nine cysteines that might be good for improvement of wheat quality.The results about Glu-A3genes in wheatfurther made clear composition of LMW-GS and provide a basis for selection of parents in wheat quality breeding.

关 键 词：普通小麦近缘种 低分子量麦谷蛋白亚基 Glu-A3基因 等位变异 

分 类 号：S512.1[农业科学—作物学]