三种感染动物阳性血清与口蹄疫病毒重组非结构蛋白的反应原性  

Reactionogenicity of Foot-and-mouth Disease Virus Recombinant Non-structural Protein against the Positive Sera of Infected Bovine,Goat and Swine

作　　者：孙学强[1,2] 邵卫星[1,2] 张如民 南文龙[1] 张兴进 宫枫举 张秀娟[1] 刘爽[1] Sun Xueqiang;Shao Weixing;Zhang Rumin;Nan Wenlong;Zhang Xingjin;Gong Fengju;Zhang Xiujuan;Liu Shuang(China Animal Health and Epidemiology Center,Qingdao,Shandong　266032;Qingdao Regen Diagnostics Development Center,Qingdao,Shandong　266114;Zhengzhou Technical College,Zhengzhou,Henan　450121)

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]青岛立见诊断技术发展中心,山东青岛266114 [3]郑州职业技术学院,河南郑州450121

出　　处：《中国动物检疫》2017年第7期92-95,107,共5页China Animal Health Inspection

基　　金：科技部科技基础性工作专项(2012FY111000)

摘　　要：本试验通过生物信息学技术比对分析GenBank中口蹄疫病毒(A型、O型和亚洲1型)非结构蛋白的保守序列,根据大肠杆菌密码子偏好性,优化合成了非结构蛋白基因,经过测序和转化,筛选到能够表达重组非结构蛋白的阳性重组菌株。经过Western-blot试验,证明表达的重组非结构蛋白能分别与口蹄疫病毒感染的牛、羊和猪阳性血清发生特异性反应。该重组蛋白待进一步验证后,可用于口蹄疫病毒感染动物的鉴别检测试剂盒研究。The conserved non-structural protein(NSP)sequence of Foot-and-mouth disease virus(type A,type O and type Asia1)was confirmed by blastp from GenBank.The DNA sequence coding the truncated conserved NSP was optimized according to the bias of E.coli.It was synthesized and cloned to expression vector.After sequencing and transformation,the positive recombinant strains expressing recombinant NSP were screened.Using western-blot,it was confirmed that the recombinant truncated NSP could react against with the positive sera of infected bovine,goat and swine.So that,the recombinant truncated NSP could be used as an antigen to develop the DIVA diagnostics kits.

关 键 词：口蹄疫病毒 感染血清 重组非结构蛋白 反应原性 

分 类 号：S852.65[农业科学—基础兽医学]