链脲佐菌素糖尿病小鼠睾丸内生精细胞减少与PCNA和Ki67表达降低  

Decreased spermatogenic cell number and expression of PCNA and Ki67 in streptozotocin-induced diabetic mice

作　　者：樊芙蓉[1] 张夔鸣[2] 唐兆生[3] 朱珍[4] 华燕菲[4] 肖天羽[4] 汪琳[5] Fan Furong;Zhang Kuiming;Tang Zhaosheng;Zhu Zhen;Hua Yanfei;Xiao Tianyu;Wang Lin(Department of Plastic Surgery ShanghaiDongfang Hospital, Shanghai 200120;Department of Neurosurgery ShanghaiDongfang Hospital, Shanghai 200120;Department of Endocrinology Shanghai Dongfang Hospital, Shanghai 200120;Department of Pathology, Shanghai Dongfang Hospital, Shanghai 200120;Department of Histology and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China)

机构地区：[1]上海市东方医院整形外科,上海200120 [2]上海市东方医院神经外科,上海200120 [3]上海市东方医院内分泌科,上海200120 [4]上海市东方医院病理科,上海200120 [5]武汉大学基础医学院组织学与胚胎学教研室,武汉430071

出　　处：《中国组织化学与细胞化学杂志》2017年第3期212-215,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的通过检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)及Ki67在正常及糖尿病小鼠睾丸组织中表达的差异,探讨糖尿病对生精细胞增殖的影响。方法 30只正常雄性C57BL/6小鼠,随机分为对照组(10只)和糖尿病组(20只)。采用腹腔注射链脲佐菌素的方法建立糖尿病小鼠模型,对照组注射相同体积的柠檬酸—柠檬酸钠缓冲液。造模成功3周后处死动物,取睾丸组织,常规固定、石蜡包埋、切片HE染色观察睾丸组织的形态学变化;免疫组织化学SP法检测PCNA及Ki67在睾丸组织中的表达;图像分析技术分析组织中PCNA及Ki67免疫组织化学表达水平。结果 HE染色显示,糖尿病小鼠睾丸内处于精子发生前半期的生精小管内生精细胞排列疏松,细胞层数偏少,附睾管内精子密度相对较低;免疫组织化学染色显示,PCNA和Ki67在糖尿病小鼠睾丸生精小管中的表达均明显降低。结论高糖可能通过降低生精细胞的增殖进而影响睾丸精子的发生。Objective To investigate the expression of proliferating cell nuclear antigen(PCNA)and Ki67in the testis of normal and diabetic mice so as to study the effect of diabetes on the proliferation of spermatogenic cells.Methods Thirty normal male C57BL/6mice were randomly divided into a control group(n=10)and a DM group(n=20).The diabetic model was established by intraperitoneal injection of streptozotocin into C57BL/6mouse,while the same volume of citric acid-sodium citrate buffer was injected in the control group mice.The mice were killed three weeks after model establishment,the testes and epididymes were sampled,fixed,then embedded in paraffin,sectioned,stained by HE,and the morphological changes were observed under microscope.The immunohistochemical SP method and image analysis technique were employed to detect and analyze the expression of PCNA and Ki67in the testis tissue.Results HE staining showed that in the diabetic group,the germ cells at the earlier stage of the spermatogenic cycle were loosely arranged in the seminiferous tubule,the number of cell layers was decreased,and the sperm density in the ductus epididymidis was relatively low.Immunohistochemical staining results showed that the expression of PCNA and Ki67was significantly lower in the diabetic group than in the normal group(P<0.01).Conclusion High glucose concentration may impact spermatogenesis by inhibiting the proliferation of spermatogenic cells.

关 键 词：生精细胞 增殖细胞核抗原 KI67 糖尿病 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]